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本文采用ＨＣＶＮＳ３区单克隆抗体、ＬＳＡＢ法检测了６５例急、慢性丙型肝炎患者肝组织中ＨＣＶＮＳ３抗原的表达状况，并对其与病理组织学活动性的关系进行了探讨，同时也检测了肝组织中的ＨＣＶＲＮＡ，显示其与ＨＣＶＮＳ３抗原的检出有较好的一致性。在急性丙型肝炎患者组，ＨＣＶＮＳ３抗原多呈胞浆型表达，且与病理组织学活动性正相关，而在慢性丙型肝炎，较多见胞核型表达，与组织学活动性并无明显关联。实验结果支持急性丙型肝炎的肝损害与ＨＣＶ的直接作用及免疫反应有关，而慢性丙型肝炎则以免疫反应引起肝损害为主。
In this paper, HCVNS3 monoclonal antibody, LSAB method was used to detect the expression of HCV NS3 antigen in liver tissues of 65 patients with acute and chronic hepatitis C, and its relationship with pathological activity was also explored, and also detected the liver HCVRNA in tissues showed a good agreement with the detection of HCV NS3 antigen. In acute hepatitis C group, HCV NS3 antigen was mostly cytoplasm-positive and positive correlated with histopathological activity, whereas in chronic hepatitis C, the expression of HCV NS3 was more obvious than that of histopathology Associated. Experimental results support the acute hepatitis C liver damage and HCV direct effects and immune response, while chronic hepatitis C is caused by immune response to liver damage based.