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目的：分析喉癌Ｂｃｌ－２基因表达及其与放疗敏感性的相互关系。方法：采用抗Ｂｃｌ－２基因蛋白单克隆抗体，采用免疫组织化学ＤＡＣＯＣＳＡＳｙｓｔｅｍ法染色技术，对７３例喉鳞癌组织标本进行分析研究。通过χ２检验判定喉癌放疗敏感组与放疗非敏感组间的Ｂｃｌ－２基因表达具有明显差异。通过Ｃｒａｍｅｒ（修正）列联系数（Ｃ）进行检验，说明喉癌放疗敏感性与Ｂｃｌ－２基因表达强度间有明显相关性。结果：可将Ｂｃｌ－２基因表达分为强度表达，中度表达及阴性表达。基因表达与放疗敏感性间有明显相关性（Ｐ＜０．０１）。随表达增强喉癌放疗敏感性相应降低。结论：可以把喉癌Ｂｃｌ－２基因表达作为喉癌放射治疗预后判定的可靠指标之一，并作为临床医生选择治疗方案的参考指标。
Objective: To analyze the relationship between Bcl-2 gene expression and radiosensitivity in laryngeal carcinoma. Methods: Using anti-Bcl-2 monoclonal antibody, immunohistochemistry DACOCSASystem staining technique was used to analyze 73 cases of laryngeal squamous cell carcinoma. By χ2 test to determine the radiotherapy of laryngeal sensitive group and non-radiosensitive Bcl-2 gene expression between the significant differences. By Cramer (Correction) column coefficient (C) test, indicating the radiosensitivity of laryngeal cancer and Bcl-2 gene expression intensity between the significant correlation. Results: Bcl-2 gene expression can be divided into intensity expression, moderate expression and negative expression. There was a significant correlation between gene expression and radiosensitivity (P <0.01). With the expression of enhanced sensitivity of laryngeal cancer, a corresponding reduction. Conclusion: Bcl-2 gene expression in laryngeal cancer can be used as a reliable indicator of the prognosis of laryngeal cancer radiotherapy and as a reference index for clinicians to choose the treatment plan.