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目的研究补骨脂素对MCF-7/ADR耐药细胞逆转作用及对耐药细胞内Ca2 +浓度影响,探讨其可能作用机制。方法采用MTT法测定补骨脂素对细胞增殖抑制作用,高效液相色谱法检测细胞内ADR的浓度,激光共聚焦显微镜测定细胞内不同时段的Ca2 +浓度。结果补骨脂素在1 ~20μmol/L浓度下能不同程度降低ADR对MCF-7/ADR细胞的IC50,并不同程度提高细胞内ADR浓度;1 ~20μmol/L补骨脂素在不同作用时段(24、48、96h)对MCF-7/ADR细胞内Ca2 +浓度与作用时间呈负相关。结论补骨脂素能逆转MCF-7/ADR细胞的MDR,其作用机制与影响耐药细胞内Ca2 +浓度,故而增加细胞内ADR浓度有关。
Objective To study the effect of psoralen on the reversal of MCF-7/ADR-resistant cells and its effect on the intracellular Ca2 + concentration in drug-resistant cells, and to explore the possible mechanism. Methods The inhibitory effect of psoralen on cell proliferation was measured by MTT assay. The concentration of ADR in cells was detected by high performance liquid chromatography. Concentration of Ca2 + in cells at different time points was determined by confocal laser scanning microscopy. Results Psoralen can reduce the IC50 of ADR to MCF-7/ADR cells at different concentrations from 1 to 20 μmol/L, and increase the intracellular ADR concentration to varying degrees; 1 to 20 μmol/L of psoralen at different time periods. (24, 48, and 96h) There was a negative correlation between the concentration of Ca2 + in MCF-7/ADR cells and the duration of action. Conclusion Psoralen can reverse the MDR of MCF-7/ADR cells, and its mechanism of action is related to the concentration of Ca2 + in drug-resistant cells, which is related to the increase of intracellular ADR concentration.