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目的掌握十堰市城区家蝇对不同杀虫剂的抗药性状况及其变化,选用比较敏感的杀虫剂减少对环境的污染,同时为正确使用杀虫剂提供科学依据[1]。方法用乙醚麻醉后,采用微量点滴器点滴1 ul杀虫剂于试虫(18~20 mg雌蝇)中胸背板上,每组试虫30只,每种杀虫剂不少于6个浓度,设丙酮为对照组,24 h后观察死亡情况。结果共测定18种杀虫剂,家蝇对菊酯类杀虫剂最敏感,溴氰菊酯LD50为0.0 116 ug/雌蝇(2011年);滴滴涕停用多年,家蝇已处于较敏感状态;混配药物敌+氯、敌+嗅敏感性较高;家蝇对有机磷杀虫剂已产生了抗药性,双硫磷LD50为3.9 506 ug/雌蝇(2013年)。结论对蝇类防制应采取综合性防制措施,以环境治理为主,合理使用杀虫剂,使用敏感性混配药物,杀虫剂交替使用,从而减缓蝇类抗药性的产生和发展。
Objective To understand the resistance status of house flies to different insecticides and their changes in Shiyan City. The more sensitive insecticides were selected to reduce the environmental pollution and provide a scientific basis for the correct use of insecticides [1]. Methods After anesthesia with ether, 1 ul insecticide was dripped on the thoracodorsalis of test insects (18-20 mg female flies) with a microdialyser, with 30 insecticides per group and no less than 6 insecticides per group Concentration, set acetone as the control group, observed after 24 h of death. Results A total of 18 insecticides were determined. Musca domestica was most sensitive to pyrethroid insecticides. Deltamethrin LD50 was 0.0 116 ug / female flies (2011). DDT was discontinued for many years and housefly was in a more sensitive state ; Mixed ene + chlorine, enemy + sniff higher sensitivity; houseflies have developed resistance to organophosphate insecticides, diphtheria LD50 3.9 506 ug / female flies (2013). Conclusion The prevention and control of flies should take integrated control measures, mainly environmental management, rational use of insecticides, the use of sensitive mixed drugs and insecticides, so as to reduce the emergence and development of flies resistance.