论文部分内容阅读
为筛选出与黄瓜抗枯萎病相关基因连锁的SSR分子标记,以对黄瓜抗枯萎病为单显性基因的母本WIS2757和感枯萎病父本津研2号及其F1、F2分离群体为试材,通过构建抗、感池对黄瓜枯萎病抗性进行SSR分析。结果表明:通过对278对SSR引物进行筛选,获得在双亲间存在多态性的引物102对,进一步通过抗、感池筛选,获得抗感池DNA间有差异的引物10对。经F2感病群体验证,共获得9个与黄瓜枯萎病抗性基因(Foc-4)连锁的SSR分子标记,并初步将Foc-4基因定位在2号染色体两标记SSR17631和SSR00684之间,距基因Foc-4的遗传距离分别为1.0 c M和0.9 c M,为进一步开发抗枯萎病分子标记及克隆黄瓜抗枯萎病基因奠定了基础。
In order to screen SSR molecular markers linked to Fusarium wilt resistance genes in cucumber, WIS2757, which is the dominant single gene of cucumber wilt resistance, and the male parent Jinyan 2 The resistance to cucumber fusarium wilt was detected by SSR analysis. The results showed that 102 pairs of primers with polymorphisms between parents were obtained by screening 278 pairs of SSR primers, and further 10 pairs of primers were obtained by resistance and pool selection. A total of 9 SSR markers linked to the Foc-4 resistance gene were obtained from the F2 susceptible population, and the Foc-4 gene was initially mapped between the two markers SSR17631 and SSR00684 on chromosome 2 The genetic distance of Foc-4 gene was 1.0 c M and 0.9 c M respectively, which laid the foundation for the further development of molecular markers against Fusarium wilt and the cloning of Fusarium wilt resistance genes in cucumber.