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目的探讨雷洛昔芬(RAL)对体外培养的前列腺基质细胞增殖和凋亡以及TGF-β1和bFGF表达的影响。方法以10~(-10)-10~(-5)mol/L浓度的RAL作用于体外培养的前列腺基质细胞,应用MTT法和TUNEL法分别检测细胞的增殖活性和凋亡率,免疫荧光染色检测细胞中TGF-β1和bFGF表达。结果 1×10~(-8)-1×10~(-5)mol/L浓度的RAL具有浓度依赖抑制前列腺基质细胞增殖(r=-0.988,P=0.002)和促凋亡(r=0.913,P=0.030)作用(P<0.05),1×10~(-9)-1×10~(-5)mol/L浓度的RAL可浓度依赖性的上调TGF-β1(r=0.986,P=0.002)和下调bFGF(r=-0.992,P=0.001)在前列腺基质细胞的表达(P<0.05),且细胞增殖活性和凋亡率的变化均与TGF-β1和bFGF的表达改变呈线性相关(P<0.05)。结论 RAL抑制培养的前列腺基质细胞增殖和促细胞凋亡,可能与上调TGF-β1和下调bFGF表达有关。
Objective To investigate the effects of raloxifene (RAL) on the proliferation and apoptosis of prostatic stromal cells and the expression of TGF-β1 and bFGF in vitro. Methods Prostate stromal cells cultured in vitro were treated with 10 ~ (-10) -10 ~ (-5) mol / L RAL. The proliferation and apoptosis of cells were detected by MTT assay and TUNEL assay respectively. Immunofluorescence staining The expression of TGF-β1 and bFGF in the cells was examined. Results RAL with concentration of 1 × 10 -8 -8 × 10 -5 mol / L inhibited proliferation of prostatic stromal cells in a concentration-dependent manner (r = -0.988, P = 0.002) and pro-apoptotic rate (r = 0.913 (P <0.05). RAL at concentrations of 1 × 10 -9 -9 × 10 -5 mol / L could up-regulate TGF-β1 (r = 0.986, P = 0.002) and downregulated bFGF (r = -0.992, P = 0.001) in prostatic stromal cells (P <0.05), and the changes of cell proliferation activity and apoptosis rate were linear with the changes of TGF-β1 and bFGF expression Related (P <0.05). Conclusion RAL can inhibit the proliferation and apoptosis of cultured prostatic stromal cells, which may be related to the up-regulation of TGF-β1 and the down-regulation of bFGF expression.