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为提高大豆的耐旱性,本研究根据大豆偏爱的密码子将来源于枯草杆菌抗旱相关基因cspB序列进行了优化,并构建了植物表达载体pCAMBIA3301-cspB,利用农杆菌介导法将cspB基因导入大豆栽培品种Bert中。用于遗传转化子叶节外植体共3 800个,经PCR和Southern鉴定,并结合PPT抗性筛选,共获得了95棵阳性转基因植株,转化率为2.5%。经初步筛选,获得了7份耐旱性较好的转基因大豆新材料。这些材料将进一步用于耐旱转基因大豆新品种培育工作。
In order to improve the drought tolerance of soybean, we optimized the cspB sequence of drought-related genes derived from Bacillus subtilis according to the preferred codons of soybean and constructed the plant expression vector pCAMBIA3301-cspB. The cspB gene was introduced into Agrobacterium tumefaciens Soybean cultivars Bert. A total of 3 800 explants of the cotyledonary node were obtained. 95 positive transgenic plants were obtained by PCR and Southern analysis combined with PPT resistance screening. The transformation rate was 2.5%. After preliminary screening, 7 new transgenic soybean materials with better drought tolerance were obtained. These materials will be further used to cultivate a new variety of drought-tolerant transgenic soybeans.