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本世纪初开始在动物组织里显示氧化酶以来,酶组织化学方法成为探索细胞生命过程中的个别代谢功能的研究手段。随着生物化学、合成化学、电子显微技术、示踪技术、免疫学技术的发展,组织细胞内显示酶的方法和理论演变成独立的酶细胞化学学科。它是临床医学、遗传学、分子生物学的重要基础学科。这学科在血液学领域里的进展极为迅速。因为临床血液学出于探索更为锐敏的病型分类手段和鉴別诊断方法,对酶细胞化学寄与极大的企望;血液组织最容易制备单离细胞标本,最容易分离不同细胞群和显示酶活性。因此结合血液细胞学发展我国的酶细胞化学学科具有极大的前景。
Since the beginning of this century, oxidase has been shown in animal tissues, and enzymatic histochemistry has become a means of exploring individual metabolic functions in the life of cells. With the development of biochemistry, synthetic chemistry, electron microscopy, tracer technology and immunology technology, the methods and theories of intracellular display of enzymes evolve into independent enzyme cell chemistry disciplines. It is an important basic disciplines in clinical medicine, genetics and molecular biology. This discipline is making tremendous progress in the area of hematology. Because of clinical hematology for the exploration of more acute type of classification methods and differential diagnosis methods, the great hope of the enzyme cell chemistry; blood tissue is the most easy to prepare single cell samples, the most easy to separate different cell populations and display enzymes active. Therefore, the combination of hematology development of China’s enzyme cell chemistry has great prospects.