论文部分内容阅读
本文以手持式γ探测仪对荷人结肠癌细胞系Ｌｓ１７４Ｔ裸鼠进行体表放射免疫探测，为临床应用放射免疫引导外科手术（ＲＩＧＳ）提供了依据。以人结肠癌细胞系Ｌｓ１７４Ｔ免疫ＢＡＬＢ／ｃ小鼠制备ＭｃＡｂＣＬ－３，胃蛋白酶消化法制备ＣＬ－３Ｆ（ａｈ′）２片段，Ｉｏｄｏｓｅｎ法以１２５Ｉ标记ＣＬ－３及其Ｆ（ａｂ′）２。两组荷瘤裸鼠注射标记物后分别于２４、４８、７２、９６、１２０、１４４和１６８ｈ以手持式γ探测仪（ＨＨＧＤ－Ｉ型）进行体表探测，测定Ｔ／Ｎ比值。１２５ＩＣＬ－３组Ｔ／Ｎ比值在１．２２～３．７９之间，第１６８小时最高；１２５ＩＣＬ－３Ｆ（ａｂ′）２组Ｔ／Ｎ比值在２．０～４．５之间，第７２～１４４小时最高。结果表明，荷瘤裸鼠腹注腔射１２５ＩＣＬ－３及１２５ＩＣＬ－３Ｆ（ａｂ′）２后标记物能特异性地浓集于肿瘤组织，以手持式γ探测仪进行体表探测Ｔ／Ｎ比值有明显意义，能明确定位肿瘤。Ｆ（ａｂ′）２用于ＲＩＧＳ裸鼠实验效果优于完整抗体。
In this paper, surface radioimmunoassay of human colon cancer cell line Ls174T in nude mice was carried out with a hand-held gamma detector, which provided the basis for the clinical application of radioimmunoguided surgery (RIGS). McAbCL-3 was prepared by immunizing BALB/c mice with human colon cancer cell line Ls174T. CL-3F (ah’)2 fragments were prepared by pepsin digestion and CL-3 and F(ab’)2 were labeled by 125I with Iodosen method. After the two groups of tumor-bearing nude mice were injected with markers, they were probed with a hand-held gamma detector (HHGD-I type) at 24, 48, 72, 96, 120, 144, and 168 hours to determine the T/N ratio. The ratio of T/N in the 125ICL-3 group was between 1.22 and 3.79, the highest at the 168th hour; the T/N ratio of the 125ICL-3F(ab’)2 group was between 2.0 and 4.5, the 72nd ~144 hours highest. The results showed that 125ICL-3 and 125ICL-3F(ab’)2 post-abdominal injections in tumor-bearing nude mice can be specifically concentrated in tumor tissues, and the body surface detection T/N ratio can be measured with a hand-held gamma detector. There is a clear meaning that can clearly locate the tumor. F(ab’)2 is more effective than intact antibody in RIGS nude mice.