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本文以手持式γ探测仪对荷人结肠癌细胞系Ls174T裸鼠进行体表放射免疫探测,为临床应用放射免疫引导外科手术(RIGS)提供了依据。以人结肠癌细胞系Ls174T免疫BALB/c小鼠制备McAbCL-3,胃蛋白酶消化法制备CL-3F(ah′)2片段,Iodosen法以125I标记CL-3及其F(ab′)2。两组荷瘤裸鼠注射标记物后分别于24、48、72、96、120、144和168h以手持式γ探测仪(HHGD-I型)进行体表探测,测定T/N比值。125ICL-3组T/N比值在1.22~3.79之间,第168小时最高;125ICL-3F(ab′)2组T/N比值在2.0~4.5之间,第72~144小时最高。结果表明,荷瘤裸鼠腹注腔射125ICL-3及125ICL-3F(ab′)2后标记物能特异性地浓集于肿瘤组织,以手持式γ探测仪进行体表探测T/N比值有明显意义,能明确定位肿瘤。F(ab′)2用于RIGS裸鼠实验效果优于完整抗体。
In this paper, surface radioimmunoassay of human colon cancer cell line Ls174T in nude mice was carried out with a hand-held gamma detector, which provided the basis for the clinical application of radioimmunoguided surgery (RIGS). McAbCL-3 was prepared by immunizing BALB/c mice with human colon cancer cell line Ls174T. CL-3F (ah’)2 fragments were prepared by pepsin digestion and CL-3 and F(ab’)2 were labeled by 125I with Iodosen method. After the two groups of tumor-bearing nude mice were injected with markers, they were probed with a hand-held gamma detector (HHGD-I type) at 24, 48, 72, 96, 120, 144, and 168 hours to determine the T/N ratio. The ratio of T/N in the 125ICL-3 group was between 1.22 and 3.79, the highest at the 168th hour; the T/N ratio of the 125ICL-3F(ab’)2 group was between 2.0 and 4.5, the 72nd ~144 hours highest. The results showed that 125ICL-3 and 125ICL-3F(ab’)2 post-abdominal injections in tumor-bearing nude mice can be specifically concentrated in tumor tissues, and the body surface detection T/N ratio can be measured with a hand-held gamma detector. There is a clear meaning that can clearly locate the tumor. F(ab’)2 is more effective than intact antibody in RIGS nude mice.