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目的建立孕兔宫内窘迫模型,探讨胎兔脑一氧化氮(NO)、诱生型一氧化氮合酶(iNOS)和总超氧化物歧化酶(T-SOD)含量变化规律。方法新西兰大白孕兔30只,随机分为空白对照组(n=12)、假手术组(n=9)和宫内缺血缺氧组(n=9),宫内缺血缺氧组建立孕兔开腹子宫动脉结扎胎兔宫内窘迫缺血缺氧模型,分别开腹取各组胎兔脑匀浆液测定NO、iNOS和T-SOD含量,进行统计学比较。结果与空白对照组比较,假手术组和宫内缺血缺氧组NO、iNOS的含量显著升高(P<0.01),T-SOD的含量明显降低(P<0.01),差异具有统计学意义。与假手术组相比,宫内缺血缺氧组NO、iNOS显著升高(P<0.01),差异具有统计学意义;T-SOD降低不显著(P=0.399)。结论通过检测孕兔宫内窘迫模型NO、iNOS和T-SOD显示,胎兔缺氧应激反应强烈,手术操作及子宫动脉结扎对脑组织内自由基含量影响显著。
Objective To establish a model of intrauterine distress in pregnant rabbits to investigate the changes of cerebral nitric oxide (NO), inducible nitric oxide synthase (iNOS) and total superoxide dismutase (T-SOD) in fetus and rabbit. Methods Thirty pregnant New Zealand white rabbits were randomly divided into blank control group (n = 12), sham operation group (n = 9) and intrauterine ischemia and hypoxia group (n = 9) Rabbits laparotomy uterine artery ligation fetus intrauterine fetal distress ischemia and hypoxia model, respectively, fetus were taken from each group of fetal brain homogenates were measured NO, iNOS and T-SOD levels were compared statistically. Results Compared with the blank control group, the contents of NO and iNOS in the sham operation group and the intrauterine ischemia / hypoxia group were significantly increased (P <0.01) and the content of T-SOD was significantly decreased (P <0.01), the difference was statistically significant . Compared with sham operation group, NO and iNOS in intrauterine ischemia and hypoxia group were significantly increased (P <0.01), the difference was statistically significant; T-SOD decreased insignificantly (P = 0.399). Conclusion The detection of NO, iNOS and T-SOD in the model of pregnancy-induced intrauterine distress showed that the hypoxia stress of the fetus was strongly influenced by the operation and the uterine artery ligation had a significant effect on the content of free radicals in the brain tissue.