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[目的]研究分析肝硬化患者外周血CD14+CD16+单核细胞分泌TNF-α、IL-10的功能特点。[方法]采用流式细胞术检测不同浓度的LPS刺激外周血单核细胞分泌细胞因子TNF-α及IL-10水平。[结果]肝硬化患者外周血单核细胞分泌TNF-α水平增加,失代偿期肝硬化组TNF-α、IL-10的水平随着LPS浓度的增加升高不明显。[结论]肝硬化患者肝脏储备功能越差,则CD14+CD16+单核细胞频率越高,促炎因子增加,其频率及生物学功能与病情的严重性有密切关系。
[Objective] To investigate the functional characteristics of TNF-α and IL-10 secreted by peripheral blood CD14 + CD16 + monocytes in patients with cirrhosis. [Method] Flow cytometry was used to detect the secretion of cytokines TNF-α and IL-10 by peripheral blood mononuclear cells under different concentrations of LPS. [Results] The levels of TNF-α secreted by peripheral blood mononuclear cells increased in patients with cirrhosis. The levels of TNF-α and IL-10 in the decompensated cirrhosis group were not significantly increased with the increase of LPS concentration. [Conclusion] The poorer liver reserve function in patients with liver cirrhosis, the higher the frequency of CD14 + CD16 + monocytes and the increase of proinflammatory cytokines, the frequency and biological function are closely related to the severity of the disease.