Control of shock wave and boundary layer interaction finds still a lot of attention. Methods of this interaction control have been especially investigated in recent decade. This research was mostly concerned with flows without separation. However, in many applications shock waves induce separation often leads to strong unsteady effects. In this context it is proposed to use streamwise vortices for the interaction control. The results of experimental investigations are presented here. The very promising results were obtained, meaning that the incipient separation was postponed and the separation size was reduced for the higher Mach numbers. The decrease of the RMS of average shock wave oscillation was also achieved. Control of shock wave and boundary layer interaction finds still a lot of attention. Methods of this interaction control have been especially investigated in recent decade. However, in many applications shock waves induce separation often leads to In this context it is proposed to use streamwise vortices for the interaction control. The very promising results were obtained, meaning that the incipient separation was postponed and the separation size was reduced for the higher decrease in the RMS of average shock wave oscillation was also achieved.