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AIM:To investigate the prognostic value of the expressionof connexin(Cx)26,32 and 43 messenger RNA(mRNA)inhepatocellular carcinoma(HCC)tissues.METHODS:Using a reverse-transcriptase polymerase chainreaction(RT-PCR),Cx 26,Cx 32 and Cx 43 mRNAs weredetermined in the liver tissues of 15 controls and in HCCtissues of 25 patients undergoing curative hepatic resection.The patients were followed up clinically.RESULTS:Cx 26 and Cx 32 mRNAs were significantly lowerin HCC tissues compared with controls(both P<0.01).Bymultivariate analysis,a lower level of Cx 26 and Cx 32mRNA correlated significantly with a risk of HCC recurrence(P=0.033)and recurrence-related mortality(P=0.031,P=0.031).Cx 43 mRNA was higher in HCC tissues comparedwith controls but did not correlate with postoperativerecurrence or recurrence-related mortality.Other significantpredictors of HCC recurrence included cellular dedifferentiation(P=0.033),less encapsulation(P=0.050),vascularpermeation(P=0.046),and daughter nodules(P=0.046).Significant variables related to recurrence-related mortalityconsisted of cell dedifferentiation(P=0.031),vascularpermeation(P=0.048),and daughter nodules(P=0.048).The levels of Cx 26 and Cx 32 mRNAs correlated significantlywith cell differentiation(P=0.031).CONCLUSION:A low expression of Cx 26 and Cx 32 mRNAsin HCC tissues is predictive of postoperative recurrence ofHCCs.
AIM: To investigate the prognostic value of the expression of connexin (Cx) 26,32 and 43 messenger RNA (mRNA) in hepatocellular carcinoma (HCC) tissues. METHODS: Using a reverse-transcriptase polymerase chain reaction 32 and Cx 43 mRNAs were confirmed in the liver tissues of 15 controls and in HCC patients of 25 patients undergoing curative hepatic resection. The patients were followed up clinically. RESULTS: Cx 26 and Cx 32 mRNAs were significantly lower in HCC tissues compared with controls (both P <0.01) .Bymultivariate analysis, a lower level of Cx 26 and Cx 32 mRNA correlated significantly with a risk of HCC recurrence (P = 0.033) and recurrence-related mortality (P = 0.031, HCC tissues compared with controls but did not correlate with postoperativerecurrence or recurrence-related mortality. Significant predictors of HCC recurrence included cellular dedifferentiation (P = 0.033), less encapsulation (P = 0.050), vascular performance (P = 0.046), and daughter nodules = 0.04 6) .Significant variables related to recurrence-related mortality associated with cell differentiation (P = 0.031), vascular performance (P = 0.048), and daughter nodules P = 0.031) .CONCLUSION: A low expression of Cx 26 and Cx 32 mRNAs HCC tissues are predictive of postoperative recurrence of HCCs.