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目的研究核黄素光化学法对乙肝病毒的灭活效果。方法随机选择慢性乙型肝炎患者15例,抽取其静脉血并使用核黄素光化学法进行病毒灭活,评价灭活效果;检测灭活前后红细胞功能。结果以1600μmol/L核黄素灭活2 h,HBV DNA拷贝数明显减少(P<0.05);灭活后红细胞Na+K+-ATPase有所降低,丙二醛(MDA)明显增高(P<0.05),而2,3-二磷酸甘油酸(2,3-DPG)、游离血红蛋白(FHb)、渗透脆性等无明显变化。结论核黄素光化学法能部分灭活血液中乙肝病毒,对红细胞功能无明显影响。
Objective To study the inactivation effect of riboflavin photochemical method on hepatitis B virus. Methods Fifteen patients with chronic hepatitis B were randomly selected, their venous blood was extracted and the virus was inactivated by riboflavin photochemical method to evaluate the inactivation effect. The function of erythrocytes before and after inactivation was detected. Results After inactivation with 1600 μmol / L riboflavin for 2 h, the copy number of HBV DNA was significantly decreased (P <0.05). After inactivation, erythrocytes Na + K + -ATPase was decreased and malondialdehyde (MDA) ), While 2,3-diphosphoglycerate (2,3-DPG), free hemoglobin (FHb), osmotic fragility and other no significant changes. Conclusion Riboflavin photochemical method can partially inactivate hepatitis B virus in the blood without any significant effect on erythrocyte function.