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选用7.5％DMSO为冷冻保护剂。降温和复温参数如下:快降温>100℃/分;慢降温1～2℃/分;快复温2>100℃/分;慢复温10℃/分。保存结束后,作胰组织四唑盐还原反应和ATP含量测定以判断胰组织活力。同时作电镜形态学观察。实验结果提示:冷冻保存结束后,慢降温快复温胰组织ATP含量及四唑盐还原反应出现蓝色的时间均优于其余三组(P<0.01)。电镜观察该组的损伤较轻而其余三组较重。作者认为选用慢降温(1～2℃/分),快复温(>100℃/分)能获得较好的冷冰保存效果。
Choose 7.5% DMSO as cryoprotectant. Cooling and rewarming parameters are as follows: fast cooling> 100 ℃ / min; slow cooling 1 ~ 2 ℃ / min; fast rewarming 2> 100 ℃ / min; slow rewarming 10 ℃ / min. After the end of storage, the pancreatic tissue tetrazolium reduction reaction and ATP content determination to determine the activity of pancreatic tissue. At the same time for electron microscopy observation. The results of the experiment indicated that after the cryopreservation, the content of ATP in the pancreas of hypothermia and rewarming and the time of blue in the tetrazolium salt reduction reaction were all better than the other three groups (P <0.01). Electron microscopy of the group showed less damage and the remaining three groups were heavier. The authors believe that the use of slow cooling (1 ~ 2 ℃ / min), rapid rewarming (> 100 ℃ / min) can get better cold preservation effect.