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目的:检测、分析喉癌组织中支原体感染的情况,探讨支原体感染对喉癌发生、发展以及预后的影响。方法:应用免疫组织化学方法对214例喉不同病变及喉癌颈转移淋巴结组织标本中支原体感染情况进行检测,其中包括喉癌121例,喉癌前病变21例,声带息肉17例,癌旁正常喉组织14例,癌周对侧正常喉组织9例以及喉癌颈转移淋巴结32例,并对结果进行分析。结果:人喉癌组织、喉癌颈转移淋巴结组织、喉癌前病变、声带息肉、癌旁正常喉组织、癌周对侧正常喉组织中抗猪鼻支原体抗体2G10反应阳性率分别为44.63%(54/121)、34.38%(11/32)、23.81%(5/21)、17.65%(3/17)、7.14%(1/14)、0%(0/9);人喉癌组织、喉癌颈转移淋巴结组织的2G10感染阳性率明显高于其他各组(P<0.05)。中晚期喉癌(Ⅲ-Ⅳ期)中2G10感染明显高于早期喉癌(Ⅰ-Ⅱ期)[68.18%(45/66)vs16.36%(9/55),P<0.01]。伴颈淋巴结转移喉癌组织2G10阳性率为62.50%(20/32),无颈淋巴结转移喉癌阳性率为35.96%(32/89),二者之间有显著差异(P<0.01)。2G10阴性Ⅲ-Ⅳ期喉癌病例3、5年生存率分别为85.71%(18/21)及84.21%(16/19),而2G10表达阳性Ⅲ-Ⅳ期喉癌病例3、5年生存率则分别为60.0%(27/45)及58.14%(25/43),2G10表达阳性病例3、5年生存率均明显低于2G10表达阴性病例(P<0.05)。结论:人喉癌组织支原体感染率较高,支原体感染与喉癌的发生、发展以及预后有一定的相关性。
Objective: To detect and analyze the mycoplasma infection in laryngeal carcinoma and to investigate the influence of mycoplasma infection on the occurrence, development and prognosis of laryngeal carcinoma. Methods: Immunohistochemistry was used to detect the mycoplasma infection in 214 cases of different laryngeal lesions and cervical lymph node metastasis of laryngeal carcinoma, including 121 cases of laryngeal carcinoma, 21 cases of laryngeal precancerous lesions, 17 cases of vocal cord polyps, 14 cases of laryngeal tissue, 9 cases of normal laryngeal tissues of contralateral cancer and 32 cases of cervical lymph node metastasis of laryngeal carcinoma. The results were analyzed. Results: The positive rates of anti-Mycoplasma hyopneumoniae antibody 2G10 in human laryngeal carcinoma, cervical lymph node tissue of laryngeal carcinoma, precancerous lesions of larynx, vocal cord polyps, normal laryngeal tissues adjacent to cancer and normal laryngeal tissues around cancer were 44.63% (54 /121),34.38%(11/32),23.81%(5/21),17.65%(3/17),7.14%(1/14),0%(0/9); human laryngeal cancer tissue, throat The positive rate of 2G10 infection in cervical lymph node metastasis was significantly higher than that in other groups (P <0.05). 2G10 infection was significantly higher in advanced laryngeal cancer (stage Ⅲ-Ⅳ) than in early laryngeal cancer (stage Ⅰ-Ⅱ) (68.18%, 45/66, vs 16.36%, 9/55, P <0.01). The positive rate of 2G10 in laryngeal carcinoma with cervical lymph node metastasis was 62.50% (20/32), and the positive rate of cervical lymph node metastasis in laryngeal carcinoma was 35.96% (32/89), with significant difference (P <0.01). The 3-year and 5-year survival rates of 2G10-negative stage III-IV laryngeal carcinoma were 85.71% (18/21) and 84.21% (16/19), respectively. The 3-year and 5-year survival rates of 2G10- (3) The 5-year and 5-year survival rates of 2G10-positive cases were significantly lower than those of 2G10-negative cases (P <0.05). Conclusion: The infection rate of mycoplasma in human laryngeal carcinoma is high. Mycoplasma infection has a certain correlation with the occurrence, development and prognosis of laryngeal cancer.