论文部分内容阅读
目的 探讨P-选择素（P-selectin）在大鼠全脑缺血再灌注血管内皮细胞损伤过程中的作用。方法将大鼠随机分为假手术组和脑缺血30min再灌注1h、3h、6h、12h、24h、48h、72h组,动物模型采用全脑缺血模型三血管阻塞法,用免疫组化法检测脑组织缺血再灌注不同时间点FⅧ相关抗原（vWF）的表达以标记内皮细胞损伤与修复过程,同时用原位杂交方法检测P-选择素mRNA的表达变化。结果 假手术对照组未见P-选择素mRNA表达,脑缺血再灌注后1h表达出现上调,12～24h达高峰,72h仍有表达。FⅧ相关抗原在假手术组呈强阳性表达,脑缺血再灌注后3h表达下降,24～48h表达最低,72h表达开始恢复。结论 脑缺血再灌注微血管内皮细胞损伤主要发生在早期,此过程中P-选择素mRNA表达上调,说明P-选择素参与了脑缺血后内皮细胞的损伤过程。
Objective To investigate the role of P-selectin in the process of injury of vascular endothelial cells after global cerebral ischemia-reperfusion in rats. Methods The rats were randomly divided into sham operation group and reperfusion for 1h, 3h, 6h, 12h, 24h, 48h, 72h after 30min ischemia. The animal models were induced by occlusion of the whole blood vessels by three-vessel occlusion method and immunohistochemistry The expression of FⅧ-associated antigen (vWF) at different time points after ischemia-reperfusion was detected to detect the process of endothelial cell injury and repair. At the same time, the expression of P-selectin mRNA was detected by in situ hybridization. Results There was no P-selectin mRNA expression in the sham-operation control group. The expression of P-selectin mRNA was up-regulated at 1h after cerebral ischemia and reperfusion, reached the peak at 12 ~ 24h and remained at 72h. FⅧ-associated antigen was strongly expressed in sham-operated group, decreased at 3h after cerebral ischemia-reperfusion, lowest at 24-48h and recovered at 72h. Conclusions Cerebral ischemia-reperfusion injury of microvascular endothelial cells occurs mainly in the early stage. P-selectin mRNA is up-regulated in this process, indicating that P-selectin is involved in the process of endothelial cell injury after cerebral ischemia.