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采用微量量热法研究拉米夫定对3种肠道特征细菌青春双歧杆菌、痢疾志贺菌及大肠埃希菌生长代谢的影响。在不同给药剂量下,以表达功率时间曲线(热谱曲线)的特征参数生长速率常数(k)、热功率(p)、达峰时间(t)、发热量(Q)为指标,对拉米夫定影响肠道特征细菌生长代谢程度进行客观地量化评价。结果显示,拉米夫定对青春双歧杆菌的半数抑制率(IC50)为200μg·mL-1,对痢疾志贺菌的IC50大于3 000μg·mL-1,对大肠埃希菌的IC50大于6 000μg·mL-1。结果显示,拉米夫定对3种肠道特征菌群抑制效果呈现明显差异,其“敏感”程度依次为青春双歧杆菌>痢疾志贺菌>大肠埃希菌;研究提示长期口服拉夫米定可能破坏菌道菌群平衡,存在导致内毒素等增加、引发炎症、加速肝病病情恶化的潜在风险。
The effects of lamivudine on the growth and metabolism of three enteric bacteria Bifidobacterium adolescentis, Shigella dysenteriae and Escherichia coli were studied by microcalorimetry. At different doses, the parameters of growth rate (k), heat power (p), time to peak (t) and calorific value (Q) were used as parameters to express the power time curve (heat spectrum curve) Mifradine affect the intestinal bacterial growth and metabolism characteristics of an objective quantitative assessment. The results showed that the half-rate inhibition rate (IC50) of lamivudine against Bifidobacterium adolescentis was 200μg · mL-1, the IC50 of Shigella dysenteriae was over 3 000μg · mL-1, and the IC50 of Escherichia coli was over 6 000μg · mL-1. The results showed that lamivudine showed significant difference in the inhibitory effect on the three intestinal microflora. The “sensitive” degree of lamivudine was Bifidobacterium adolescentis> Shigella dysenteriae> Escherichia coli. The study suggested that long-term oral Rave Middine may destroy the balance of the bacterial flora, leading to increased risk of endotoxin and other inflammation, and accelerate the deterioration of liver disease.