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选用功能单体3-氨基苯硼酸(APBA)和乳糖酸(LA)分别对聚谷氨酸(γ-PGA)和壳寡糖(CS)接枝改性后制备聚谷氨酸-g-氨基苯硼酸(γ-PGA-g-APBA)及糖基化壳寡糖(GC);以二氧化硅微球为模板,通过γ-PGA-g-APBA和GC间的静电相互作用进行层层自组装,再经脱除模板则可获得形貌规整可控的生物基胶囊.通过红外光谱(FTIR)和核磁共振(1H NMR)对聚合物化学结构、接枝率进行表征;利用Zeta电位监测聚合物电解质层层自组装的进程,并通过透射电镜(TEM)和扫描电镜(SEM)观测胶囊的形貌.同时考察了胶囊在不同温度、盐浓度、p H值及糖浓度下的刺激响应,研究结果表明胶囊在一定的温度、盐浓度、p H值下能稳定存在;低浓度葡萄糖刺激时胶囊形貌完整,而高浓度葡萄糖刺激时,胶囊溶胀直至结构与形貌破坏,说明功能单体LA和APBA的引入可赋予胶囊具有葡萄糖响应性.这种具有良好稳定性和葡萄糖响应性的生物基胶囊有望应用于糖尿病的诊断和治疗.
Polyglutamic acid-g-amino groups were prepared by grafting polyglutamic acid (γ-PGA) and chitooligosaccharides (CS) with functional monomers 3-aminophenylboronic acid (APBA) and lactobionic acid (LA) (Γ-PGA-g-APBA) and glycosylated chitooligosaccharides (GC). Silica microspheres were used as templates for the layer self-assembly by electrostatic interaction between γ-PGA-g-APBA and GC Assembly, and then remove the template can be obtained by the morphology of the controllable bio-based capsules.The chemical structure of the polymer by chemical infrared spectroscopy (FTIR) and nuclear magnetic resonance grafting rate was characterized by Zeta potential monitoring polymerization And the morphology of the capsules was observed by transmission electron microscopy (TEM) and scanning electron microscopy (SEM). The stimulus responses of the capsules at different temperatures, salt concentrations, p H values and sugar concentrations were also investigated. The results showed that the capsule can exist stably at a certain temperature, salt concentration and p H value. The capsule morphology was intact when stimulated with low concentration of glucose. When the capsule was swollen by high concentration of glucose, the structure and morphology were destroyed, indicating that the functional monomer The introduction of LA and APBA confers glucose responsiveness to the capsule, which has good stability and glucose response Biobased capsule expected to be applied in the diagnosis and treatment of diabetes.