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目的探讨抗原处理和抗原提呈在重症肌无力患者胸腺组织T细胞异常分化中的作用。方法收集5例重症肌无力(myasthenia gravis,MG)患者(MG组)和5例非自身免疫性先天性心脏病患者(对照组)胸腺组织,提取RNA,反转录为cDNA,采用树突状细胞与抗原提呈相关基因芯片进行实时定量PCR,采用ΔΔCt方法对数据进行分析,比较2组ΔCt值。结果 2组树突状细胞和抗原提呈细胞芯片中含有84种共8类基因;MG患者胸腺组织表达水平明显增高基因有9种,包括与抗原摄取、提呈相关的TAP2、CD4、THBS1和与细胞因子及细胞因子受体相关的CCL2、CCL3、CXCL2、干扰素-γ(interferon-γ,IFN-γ)、白细胞介素-6(interleukin-6,IL-6)、ERBB2;MG组CCL2(-0.34±0.06),CCL3(3.03±0.45),CD4(-2.95±0.11),CXCL2(0.66±0.21),ERBB2(3.25±0.39),IFN-γ(3.74±0.44),IL-6(1.64±0.22),TAP2(-1.51±0.15),THBS1(0.76±0.55)基因ΔCt值与对照组(3.64±0.03、4.11±0.12、-0.56±0.05、2.26±0.86、4.53±0.48、6.37±0.87、5.76±1.06、0.05±0.01、3.83±0.76)比较差异均有统计学意义(P<0.05);9种基因MG组2-ΔΔCt/对照组2-ΔΔCt均>2。结论 MG患者胸腺组织多种抗原提呈作用相关的基因表达存在异常,可能与T细胞在胸腺的异常分化有关。
Objective To investigate the role of antigen processing and antigen presentation in the abnormal differentiation of thymus T cells in patients with myasthenia gravis. Methods Thymus tissue was collected from 5 patients with myasthenia gravis (MG) and 5 patients with non-autoimmune congenital heart disease (control group). RNA was extracted and reverse transcribed into cDNA. The dendritic Real-time quantitative PCR was performed on the gene chips related to cell-antigen presentation. The ΔΔCt method was used to analyze the data and the ΔCt values of the two groups were compared. Results The dendritic cells and antigen presenting cells in two groups contained 84 kinds of 8 genes in total. The expression levels of thymus in MG patients were significantly increased, including 9 genes including TAP2, CD4, THBS1 correlated with antigen uptake and presentation CCL2, CCL3, CXCL2, IFN-γ, IL-6 and ERBB2 related to cytokines and cytokine receptors; CCL2 (-0.34 ± 0.06), CCL3 (-2.95 ± 0.11), CXCL2 (0.66 ± 0.21), ERBB2 (3.25 ± 0.39), IFN- γ (3.74 ± 0.44) and IL-6 ± 0.22), TAP2 (-1.51 ± 0.15) and THBS1 (0.76 ± 0.55) were significantly higher than those in control group (3.64 ± 0.03, 4.11 ± 0.12, -0.56 ± 0.05, 2.26 ± 0.86, 4.53 ± 0.48, 6.37 ± 0.87, 5.76 ± 1.06,0.05 ± 0.01,3.83 ± 0.76, respectively) (P <0.05). The 2-ΔΔCt of 9 gene MG and the 2-ΔΔCt of control group were all> 2. Conclusion There is abnormal gene expression related to multiple antigen presentation in thymus of patients with MG, which may be related to the abnormal differentiation of T cells in the thymus.