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目的观察支气管哮喘(简称哮喘)大鼠及哮喘患者支气管管壁尿紧张素Ⅱ(UⅡ)的变化,探讨UⅡ在哮喘发病及气道重塑中的作用。方法将20只一级雄性Wistar大鼠分为对照组和哮喘组,每组10只。哮喘组大鼠采用卵白蛋白10mg腹腔内注射及雾化吸入(5mg)处理建立大鼠哮喘模型;肺组织切片经苏木精伊红(HE)染色,采用图像分析技术测量大鼠支气管管腔的内周长(Pi)、管壁面积(WA)、平滑肌面积(WAm),以WA/Pi和WAm/Pi反映其气道重塑情况;对哮喘大鼠和5例哮喘患者肺组织切片采用链亲和素蛋白过氧化物酶(SP)免疫组织化学法染色检测支气管UⅡ的表达变化,以灰度扫描和计数UⅡ阳性染色细胞数判断其表达强度。结果哮喘组大鼠WA/Pi、WAm/Pi分别为(24.1±2.4)μm2/μm、(5.3±1.9)μm2/μm,与对照组[(16.5±1.7)μm2/μm、(3.8±1.2)μm2/μm]比较差异有统计学意义(t分别=3.892、3.785,P均<0.01);哮喘大鼠支气管UⅡ的表达强度为2.46±0.15,与对照组(1.26±0.11)比较差异有统计学意义(t=6.236,P<0.01);UⅡ的表达强度与WAm/Pi呈正相关(r=0.712,P<0.01);UⅡ阳性染色细胞百分数为(82±8)%,与对照组[(22±8)%]比较差异也有统计学意义(t=19.102,P<0.01);哮喘患者支气管UⅡ的表达强度为2.61±0.19,与对照组(1.36±0.12)比较差异有统计学意义(t=7.374,P<0.01);UⅡ阳性染色细胞百分数为(75±9)%,与对照组[(27±7)%]比较差异有统计学意义(t=16.236,P<0.01)。结论哮喘气道UⅡ的表达和合成明显增加,其可能与哮喘发病及其气道重塑具有密切的关系。
Objective To observe the changes of bronchial wall urotensin Ⅱ (U Ⅱ) in bronchial asthma rats and asthmatic patients, and to explore the role of U Ⅱ in asthma and airway remodeling. Methods Twenty male Wistar rats were divided into control group and asthma group, with 10 in each group. The rats in the asthma group were treated with intraperitoneal injection of ovalbumin (10 mg) and aerosolized inhalation (5 mg) for the establishment of a rat model of asthma. The lung tissue sections were stained with hematoxylin and eosin (HE), and image analysis was used to measure the bronchial lumen (Pi), wall area (WA) and smooth muscle area (WAm) were measured. The airway remodeling was reflected by WA / Pi and WAm / Pi. Lung tissues of asthmatic rats and 5 asthmatic patients The expression of UⅡ in bronchi was detected by immunohistochemical staining of avidin (SP) immunohistochemical staining, and the intensity of expression of U Ⅱ was detected by gray-scale scanning and counting the number of UⅡ positive cells. Results WA / Pi and WAm / Pi in the asthma group were (24.1 ± 2.4) μm2 / μm and (5.3 ± 1.9) μm2 / μm respectively compared with those in the control group [(16.5 ± 1.7) μm2 / μm and (3.8 ± 1.2) μm 2 / μm] (t = 3.892, 3.785 respectively, P <0.01). The expression of bronchial UⅡ in asthmatic rats was 2.46 ± 0.15, which was significantly lower than that in the control group (1.26 ± 0.11) The expression of UⅡ was positively correlated with WAm / Pi (r = 0.712, P <0.01), while the percentage of UⅡ positive cells was (82 ± 8)%, which was significantly lower than that of control group [(22 (T = 19.102, P <0.01). The expression of bronchial UⅡ in asthmatic patients was 2.61 ± 0.19, which was significantly lower than that in the control group (1.36 ± 0.12) (t = 7.374, P <0.01). The percentage of UⅡpositive cells was (75 ± 9)%, which was significantly different from that of the control group (27 ± 7)%] (t = 16.236, P <0.01). Conclusion The expression and synthesis of U Ⅱ in airway of asthma increased significantly, which may be closely related to asthma and airway remodeling.