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目的应用超声造影定量分析技术评价右美托咪定(DEX)对兔肾缺血/再灌注(I/R)损伤时微循环灌注的影响。方法新西兰家兔24只随机分成3组(每组8只):对照组、肾缺血/再灌注损伤组(I/R组)和右美托咪定组(DEX组)。I/R组和DEX组切除右肾建立左肾缺血再灌注损伤模型。DEX组在肾缺血前30 min腹腔注射10μg/kg右美托咪定。再灌注24 h后,测定肾脏大小和肾血流阻力(RI),超声造影观察肾皮质灌注,时间-强度曲线定量分析达峰时间(TTP)、峰值强度(PSI)、曲线上升斜率(Grad)和曲线下面积(AUC)。取肾脏观察病理改变。结果与对照组比较,I/R组和DEX组肾大小和病理改变明显,RI增高,TTP延长,PSI和Grad降低,AUC显著增加(P<0.05);与I/R组比较,DEX组肾大小和病理改变明显改善,RI降低,TTP缩短,PSI和Grad增高,AUC减少(P<0.05)。结论超声造影结合时间-强度曲线参数能动态定量分析右美托咪定改善兔肾缺血/再灌注损伤时的微循环灌注。
Objective To evaluate the effect of dexmedetomidine (DEX) on microcirculation perfusion during renal ischemia-reperfusion (I / R) injury in rabbits by quantitative contrast-enhanced ultrasound. Methods Twenty - four New Zealand rabbits were randomly divided into three groups (n = 8): control group, renal ischemia / reperfusion injury group (I / R group) and dexmedetomidine group (DEX group). I / R group and DEX group were excised right kidney to establish the model of left renal ischemia-reperfusion injury. DEX group was injected intraperitoneally with 10 μg / kg dexmedetomidine 30 min before renal ischemia. Renal cortical perfusion was observed by contrast-enhanced ultrasound (CEUS). Time to peak (TTP), peak intensity (PSI), and Grad And area under the curve (AUC). Take the kidneys to observe the pathological changes. Results Compared with the control group, the renal size and pathological changes of I / R group and DEX group were obviously increased, RI increased, TTP prolonged, PSI and Grad decreased, AUC increased significantly (P <0.05); Compared with I / R group, The size and pathological changes were significantly improved, RI decreased, TTP shortened, PSI and Grad increased, AUC decreased (P <0.05). Conclusion Contrast-enhanced ultrasound (CEUS) combined with time-intensity curve can dynamically and quantitatively determine the effect of dexmedetomidine on microcirculation perfusion in rabbits with renal ischemia / reperfusion injury.