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用自行研配的低温保存液对泥鳅等三种鱼的心脏跳动期胚胎进行低温保存试验。试验中分别采用慢速、分段慢速及分段快速三种降温程序，保存后的胚胎又分别以在4、25和40℃的水浴中复温。从存活率和孵化率看，分段快速降温程序较好，分段慢速降温次之，慢速降温最差；而复温方式以40℃方式最佳，25℃次之，4℃最差。其中又以分段快速降温和40℃复温的效果最好。用此法经-196℃保存的胚胎有高达70％~80％的存活率。慢速降温后再以慢速复温方式保存胚胎的效果最差，即使降温到0℃，胚胎的存活率也仅10％－15％，且无一孵化出膜。此外，还对低温保存的泥鳅胚胎作了电镜观察，结果表明：分段快速降温及40℃复温保存的胚胎，其细胞内的细胞核和线粒体等细胞器的超微结构仅受到极其轻微的冰晶损伤，而慢速降温及40℃复温的胚胎，其细胞器的超微结构受到严重的冰晶损伤。
Low-temperature preservation test was carried out on the heartbeat embryos of three kinds of fish, The experiments were slow, slow and sub-segment slow three rapid cooling program, the preserved embryos were again in the 4,25 and 40 ℃ water bath rewarming. From the survival rate and hatchability rate, the rapid sub-stage cooling program is better, followed by sub-slow cooling, slow cooling is the worst; and rewarming at 40 ℃ the best way, followed by 25 ℃, 4 ℃ the worst . Among them, the rapid cooling and 40 ℃ reheating the best results. Embryos preserved by this method at -196 ° C have a survival rate of up to 70% to 80%. Slow-cooling followed by slow rewarming save embryos worst, even if the temperature dropped to 0 ℃, the survival rate of embryos is only 10% -15%, and no hatching of the film. In addition, the embryos of loach preserved at low temperature were observed by electron microscopy. The results showed that the ultrastructure of organelles such as intracellular nucleus and mitochondria were damaged only by mild ice damage , While the slow cooling and rewarming at 40 ℃ embryos, the ultrastructure of organelles suffered severe ice damage.