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目的从红树林来源的黄柄曲霉(Aspergillus flavipes)及绮丽穗霉(Spicaria elegans KLA03)的6种培养基发酵液中获得12种粗多糖并评价其抗流感病毒(H1N1)活性。方法运用高效液相色谱(HPLC),红外光谱(IR)等对各粗多糖的单糖组成和基本结构特征进行分析比较;采用犬肾(MDCK)细胞模型结合细胞病变效应(CPE)实验对其体外抗H1N1活性进行评价。结果所有粗多糖均含有甘露糖、葡萄糖和半乳糖,某些多糖还含有氨基葡萄糖、氨基半乳糖或少量木糖与阿拉伯糖;在250μg/mL的浓度下,各多糖呈现了不同的抗H1N1病毒活性,其中AF-3及SEK-4对H1N1抑制率分别达到为69.6%和67.5%。结论通过改变生长条件,从海洋真菌发酵液中得到了2种具有较强抑制H1N1活性的胞外多糖。
Objective To obtain 12 kinds of crude polysaccharides and evaluate the anti-influenza virus (H1N1) activity from 6 culture broths of Aspergillus flavipes and Sporaria elegans KLA03. Methods The monosaccharide compositions and basic structural characteristics of each polysaccharide were analyzed by high performance liquid chromatography (HPLC) and infrared spectroscopy (IR). The canine kidney (MDCK) cell model combined with cytopathic effect (CPE) In vitro anti-H1N1 activity was evaluated. Results All of the crude polysaccharides contained mannose, glucose and galactose. Some of the polysaccharides also contained glucosamine, galactosamine or a small amount of xylose and arabinose. At a concentration of 250 μg / mL, each polysaccharide exhibited a different anti-H1N1 virus The inhibitory rates of AF-3 and SEK-4 to H1N1 were 69.6% and 67.5% respectively. Conclusion Two exopolysaccharides with strong inhibitory activity on H1N1 were obtained from marine fungal fermentation broth by changing the growth conditions.