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用６０只新西兰兔分两部分进行实验。（１）用１２只制备下丘脑细胞悬液，在离体条件下，应用Ｆｕｒａ—２荧光指示剂测定细胞内Ｃａ￣（２＋）浓度（［Ｃａ￣（２＋）］ｉ）．结果表明，用ＥＧＴＡ络合神经细胞外Ｃａ￣（２＋）而降低细胞外Ｃａ２＋浓度时，下丘脑神经细胞［Ｃａ￣（２＋）］ｉ明显降低（Ｐ＜０．Ｏ１）；相反，增加细胞外Ｃ３２＋浓度，则［Ｃａ￣（２＋）］ｉ明显增高（Ｐ＜０．０１）．（２）用４８只家兔分４组，分别向侧脑室记注ＡＣＳＦ、ＥＧＴＡ、ＥＧＴＡ＋ＣａＣｌ２，和ＣａＣｌ２，观察结肠温度和下丘脑ｃＡＭＰ含量变．结果表明，侧脑室灌注ＥＧＴＡ，引起下丘脑ｃＡＭＰ含量明显增高（Ｐ＜０．００１）和结肠温度明显上升（Ｐ＜０．００１），灌注ＥＧＴＡ后立即灌注ＣａＣｌ２，则抑制了ＥＧＴＡ引起的下丘脑ｃＡＭＰ含量增高和结肠温度上升．体温反应指数（ＴＲＩ１．５）与下丘脑ＣＡＭＰ含量呈显著正相关（ｒ＝０．８１２Ｐ＜００１）．上述结果进一步证明“下丘脑细胞内Ｃａ２＋↓→ｃＡＭＰ↑可能是ＥＧＴＡ性发热的重要中枢机制。
Sixty New Zealand rabbits were tested in two parts. (1) Hypothalamic cell suspension was prepared with 12 cells and the intracellular Ca 2+ concentration ([Ca 2+ (2 +)] i was determined with Fura-2 fluorescent indicator in vitro. The results showed that the Ca (2 +)] i of hypothalamic neurons was significantly decreased (P <0.01) when EGTA complexed with extracellular Ca ~ (2+) and decreased the extracellular Ca ~ C32 + concentration, [Ca ~ (2 +)] i was significantly higher (P <0.01). (2) Forty-eight rabbits were divided into 4 groups. ACSF, EGTA, EGTA + CaCl2 and CaCl2 were recorded in the lateral ventricle respectively. The colonic temperature and the content of cAMP in the hypothalamus were observed. The results showed that intracerebroventricular infusion of EGTA induced a significant increase in cAMP content in the hypothalamus (P <0.001) and a significant increase in colonic temperature (P <0.001). Immediate infusion of CaCl2 after EGTA infusion inhibited the hypothalamic Increased cAMP levels and colonic temperature. There was a significant positive correlation between temperature response index (TRI1.5) and hypothalamic CAMP content (r = 0.812P <001). These results further prove that "intracellular Ca2 + ↓ → cAMP ↑ hypothalamic cells may be an important central mechanism of fever.