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目的观察蒺藜甾体皂苷类化合物TTS-12对白念珠菌生物被膜形成的影响,探讨其可能的作用机制。方法采用激光共聚焦显微镜观察TTS-12对白念珠菌生物被膜生长形态的影响;采用XTT法考察不同浓度TTS-12对白念珠菌生物被膜形成的影响;应用光密度值测定及实时定量RT-PCR(real-time RT-PCR)法考察不同浓度TTS-12对白念珠菌细胞表面疏水性(cell surface hydrophobicity,CSH)及相关基因表达的影响。结果与未加TTS-12的正常对照细胞相比,经TTS-12处理的白念珠菌生物被膜结构疏松,TTS-12可剂量依赖性地抑制白念珠菌生物被膜的形成,其生长动力学及细胞表面疏水性值下降,CSH1基因表达水平降低(P<0.01)。结论TTS-12可能通过降低白念珠菌CSH1基因表达来抑制白念珠菌生物被膜的形成。
Objective To investigate the effects of the steroidal saponin compound TTS-12 on the formation of Candida albicans biofilm and to explore its possible mechanism. Methods The effect of TTS-12 on the growth of C. albicans biofilm was observed by laser confocal microscopy. The effect of TTS-12 on the formation of C. albicans biofilm was investigated by XTT method. The optical density and real-time quantitative RT-PCR Real-time RT-PCR method was used to investigate the effect of TTS-12 on the cell surface hydrophobicity (CSH) and related gene expression in Candida albicans. Results TTS-12-treated C. albicans biofilm showed a loose structure. Compared with normal control cells without TTS-12, TTS-12 inhibited the formation of C. albicans biofilm in a dose-dependent manner, and its growth kinetics and Cell surface hydrophobicity decreased, CSH1 gene expression decreased (P <0.01). Conclusion TTS-12 may inhibit the formation of C. albicans biofilm by decreasing the expression of C. albicans CSH1 gene.