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目的分析新疆伊犁河谷博尔纳病病毒(BDV)种系形成及其可能的原因。方法采用改进的巢式逆转录PCR(nRT-PCR)方法,在5个种属8个品种873例动物外周血单核细胞(PBMCs)中,检出伊犁马、新疆驴和哈萨克牧羊犬BDV磷蛋白(p24)RNA阳性片段19例,比对BDV标准株He/80、Strain V和H1766,分析核苷酸和氨基酸同源相似度,重构基因系统发生树。结果19例BDV p24核苷酸序列一部分形成伊犁独立支系,另一部分汇聚至伊犁-德国-瑞士混合支系。独立支系与标准株无聚合,核苷酸序列同源相似度为98%,氨基酸序列为100%;德国马He/80汇聚混合支系,核苷酸与氨基酸序列与He/80同源相似度均为100%。结论新疆伊犁河谷动物宿主中可能存在地源性BDV独立伊犁株,同一BDV感染不同种属动物可能源于外来疫病病原经草原丝绸之路的传播。
Objective To analyze the formation of Borna disease virus (BDV) in Yili Valley, Xinjiang and its possible causes. Methods BDNP was detected in 873 animal peripheral blood mononuclear cells (PBMCs) from 8 species and 5 species in China by using improved nested reverse transcription PCR (nRT-PCR) Nineteen positive samples of protein (p24) were compared with BDV standard strains He / 80, Strain V and H1766 to analyze the nucleotide and amino acid homology similarity and reconstruct phylogenetic tree. Results Nineteen BDV p24 nucleotide sequences formed part of the Ili independent branch and the other part of the Ili - Germany - Switzerland mixed branch. Independent branch and standard strain without polymerization, the homology similarity of nucleotide sequence is 98% and the amino acid sequence is 100%. The German He / 80 convergent mixed branch has a nucleotide and amino acid sequence homologous to He / 80 Degree are 100%. Conclusion There may be terrestrial BDV-independent Ili strains in the animal hosts of Yili River Valley in Xinjiang. The animals of the same BDV infection may be derived from the spread of the pathogen of the foreign diseases through the Silk Road.