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目的统一复方血栓通颗粒、片、滴丸和软胶囊中三七皂苷R1、人参皂苷Rg1、人参皂苷Re和人参皂苷Rb1的含量测定方法。方法采用高效液相色谱法,使用Thermo ODS2(5μm,150.0 mm×4.6 mm)色谱柱,柱温为20℃,以乙腈-水为流动相进行梯度洗脱,流速为1.0 ml/min,检测波长为203 nm。结果三七皂苷R1在0.0518~2.5900(r=0.9996)、人参皂苷Rg1在0.2090~8.3588(r=0.9991)、人参皂苷Re在0.0507~2.5371(r=0.9990)、人参皂苷Rb1在0.2044~8.1752(r=0.9995)范围内与峰面积线性关系良好;精密度、重复性、稳定性及加样回收率结果均能满足分析要求。结论该方法简单可行,为复方血栓通品种中三七皂苷R1、人参皂苷Rg1、人参皂苷Re和人参皂苷Rb1含量测定方法的统一提供了可靠的依据。
Objective To determine the content of notoginsenoside R1, ginsenoside Rg1, ginsenoside Re and ginsenoside Rb1 in Fufang Xueshuantong granules, tablets, pills and soft capsules. Methods The column of Thermo ODS2 (5μm, 150.0 mm × 4.6 mm) was used with high performance liquid chromatography (HPLC). The column temperature was 20 ℃. The mobile phase was acetonitrile - water. The flow rate was 1.0 ml / min. 203 nm. Results The results showed that the concentrations of notoginsenoside R1 at 0.0518-2.5900 (r = 0.9996), ginsenoside Rg1 at 0.2090-8.3588 (r = 0.9991), ginsenoside Re at 0.0507-2.5371 (r = 0.9990) and ginsenoside Rb1 at 0.2044-8.1752 = 0.9995) within the range of the peak area of a good linear relationship; precision, repeatability, stability and sample recovery results can meet the analysis requirements. Conclusion The method is simple and feasible, which provides a reliable basis for the unification of the methods for the determination of notoginsenoside R1, ginsenoside Rg1, ginsenoside Re and ginsenoside Rb1 in the compound Xueshuantong.