配体结合(Ligand binding)是研究受体的重要技术。大多数情况下,天然配体是已知的,如一些神经递质或多肽激素。但阿片受体是通过显示氚标记外源性吗啡类药物与脑匀浆膜的高亲和性结合而发现的,从而在推动寻找阿片受体的内源性配体-脑啡肽及内啡肽中起着决定性的作用。本实验提示,大 Ligand binding is an important technique for studying receptors. In most cases, natural ligands are known, such as some neurotransmitters or polypeptide hormones. Opioid receptors, however, have been discovered by demonstrating the high affinity of tritium-labeled exogenous morphine to brain homogenate membranes, thereby promoting the search for opioid receptors for the endogenous ligands enkephalin and endorphins Peptides play a decisive role. The experiment suggested that large