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目的探讨慢性心理应激对大鼠肠道敏感性及血浆孤啡肽(OFQ)含量的影响。方法 80只SD雌性大鼠分为实验组和对照组,实验组大鼠采用束缚应激的方法制作肠易激综合征(IBS)动物模型,并鉴定模型。2组分别于实验0、1、2、3、4周时随机取出8只,用球囊扩张的方法测定肠道敏感性,用放射免疫方法测定血浆OFQ含量。结果 IBS模型经鉴定符合其病理特征,结肠组织学观察显示各组均无明显炎症表现。实验组大鼠各时点肠道敏感性均高于相应对照组(P均<0.01);应激1周时肠道敏感性最高(P均<0.01);应激2周时,肠道敏感性高于3、4周组(P均<0.01);应激3、4周肠道敏感性差异无统计学意义(P>0.05)。实验组大鼠血浆OFQ含量与相应对照组比较均明显升高(P均<0.01),应激1周时最高(P<0.05或P<0.01),2～4周逐渐下降,3、4周时差异无统计学意义(P>0.05),但仍高于应激前水平(P均<0.05)。肠道敏感性与血浆OFQ含量变化趋势一致,二者呈显著正相关(r=0.660,P<0.01)。结论慢性心理应激引起大鼠肠道敏感性及血浆OFQ含量增高;血浆OFQ含量与肠道敏感性变化趋势一致,可能与慢性心理应激大鼠肠道敏感性变化有关。
Objective To investigate the effects of chronic psychological stress on intestinal sensitivity and plasma OFQ in rats. Methods Eighty SD female rats were randomly divided into experimental group and control group. The experimental group was induced by IBS and the model was established. Two groups were randomly selected at 0, 1, 2, 3 and 4 weeks, respectively. Eight rabbits were randomly divided into three groups. The intestinal sensitivity was measured by balloon dilatation. The content of OFQ in plasma was measured by radioimmunoassay. Results IBS model was identified in line with its pathological features, colon histological observations showed no significant inflammatory manifestations in each group. The intestinal sensitivity of rats in experimental group was higher than that of corresponding control group at each time point (P <0.01). The intestinal sensitivity was the highest at 1 week (P <0.01) (P <0.01). There was no significant difference in intestinal sensitivity between 3 and 4 weeks after stress (P> 0.05). Compared with the corresponding control group, the content of OFQ in the experimental group was significantly increased (P <0.01 or P <0.01 or P <0.01 or P <0.01 or P <0.01), and gradually decreased in 2-4 weeks (P> 0.05), but still higher than those before stress (all P <0.05). Intestinal sensitivity and plasma OFQ content trends, the two showed a significant positive correlation (r = 0.660, P <0.01). Conclusion Chronic psychological stress can cause intestinal sensitivity and increase the content of OFQ in plasma. The content of OFQ in plasma is consistent with the changes of intestinal sensitivity, which may be related to the changes of intestinal sensitivity in chronic psychological stress rats.