目的：采用ＨＰＬＣ测定心宁胶囊中葛根素含量，以控制该制剂的质量。方法：用十八烷基键合硅胶柱分离葛根素，以甲醇水磷酸（２８０∶７２０∶０．１５）为流动相，检测波长２５０ｎｍ。结果：葛根素峰与其它组分峰的分离度大于１，理论塔板数以葛根素峰计算为８３１０；线性范围０．２～２μｇ（ｒ＝０．９９９９）；平均回收率９９．４％，ＲＳＤ＝２．４７％（ｎ＝５）。结论：本法简便、快速，适用于该产品质量分析检验 Objective: To determine the puerarin content of Xinning capsule by HPLC to control the quality of the preparation. Methods: Puerarin was separated on octadecyl - bonded silica gel column with methanol  water  phosphoric acid (280:720:0.15) as mobile phase and the detection wavelength was set at 250 nm. Results: The separation of puerarin and other components was more than 1, the number of theoretical plates was 8310 for puerarin peak, the linear range was 0.2-2 μg (r = 0.9999), the average recovery was 99.4% , RSD = 2.47% (n = 5). Conclusion: This method is simple, fast, suitable for the product quality analysis test