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目的:探讨不同剂量咪唑克生(Ida)对Wistar大鼠实验性自身免疫性脑脊髓炎(EAE)中枢神经系统(CNS)内单核细胞趋化蛋白-1(MCP-1)、巨噬细胞炎性蛋白-1α(MIP-1α)表达的影响。方法:以豚鼠脊髓匀浆加完全福氏佐剂免疫Wistar大鼠,建立EAE模型。观察Ida0.5、1.5和4.5mg·kg-1剂量组对大鼠的EAE发病率、神经功能缺损评分的影响,应用苏木精-伊红和Kluver & Barrera髓鞘染色方法观察大鼠CNS病理变化,采用免疫组织化学技术检测大鼠CNS内MCP-1、MIP-1α表达的变化。结果:Ida1.5和4.5mg·kg-1组的EAE发病率和神经功能缺损评分均明显降低,Ida3个不同剂量Ida干预组的CNS内MCP-1、MIP-1α的表达呈现不同程度地减少。结论:不同剂量的Ida能改善EAE,以1.5和4.5mg·kg-1组的效果更显著。推测可能与咪唑啉2受体有关。
OBJECTIVE: To investigate the effects of different doses of Ida on the expression of monocyte chemoattractant protein-1 (MCP-1), macrophages in central nervous system (CNS) of experimental autoimmune encephalomyelitis (EAE) Effect of inflammatory protein-1α (MIP-1α) expression. Methods: Wistar rats were immunized with guinea pig spinal cord homogenate plus complete Freund ’s adjuvant to establish EAE model. To observe the effects of Ida 0.5, 1.5 and 4.5 mg · kg -1 on the incidence of EAE and neurological deficit in rats. The CNS pathology was observed by hematoxylin-eosin and Kluver Barrera myelin staining Changes in the expression of MCP-1, MIP-1α in rat CNS were detected by immunohistochemistry. Results: The incidences of EAE and neurological deficit in Ida1.5 and 4.5 mg · kg-1 groups were significantly decreased. The expression of MCP-1 and MIP-1α in CNS of 3 Ida groups with different doses of Ida decreased to some extent . Conclusion: Ida at different doses can improve EAE, and the effect is more obvious at 1.5 and 4.5 mg · kg-1. Speculated that imidazoline 2 receptors may be related.