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目的研究严重急性呼吸综合征(SARS)冠状病毒(CoV)相关受体血管紧张素转换酶2(ACE2)和 CD209L 在人8种不同器官组织微血管内皮细胞上的表达,分析两种受体表达水平与SARS 器官病变之间的相关性。方法应用反转录聚合酶链式反应(RT-PCR)、Western 印迹、免疫细胞化学3种方法分析比较 SARS-CoV 相关受体,ACE2、CD209L 在人8种不同器官组织微血管内皮细胞上的表达。结果人的各种器官组织的血管内皮细胞均表达 SARS-CoV 相关受体 ACE2与CD209L,其中 ACE2在肺微血管内皮细胞表达最高,在淋巴内皮细胞表达最低,而 CD209L 在淋巴内皮细胞表达相对较高。结论人的微血管内皮细胞是 SARS-CoV 作用的重要靶点,肺微血管内皮细胞和淋巴内皮细胞的损伤可能分别由两种不同的 SARS-CoV 受体介导。
Objective To investigate the expression of angiotensin-converting enzyme 2 (ACE2) and CD209L, a receptor of coronavirus (CoV) -related genes of severe acute respiratory syndrome (SARS), on human microvascular endothelial cells from eight different organs and to analyze the expression of two receptors And SARS organ lesion correlation. Methods The expression of SARS-CoV related receptors, ACE2 and CD209L on microvascular endothelial cells of 8 different organs were analyzed by RT-PCR, Western blot and immunocytochemistry . Results The human vascular endothelial cells of various organs expressed SARS-CoV-related receptors ACE2 and CD209L, of which ACE2 was most expressed in pulmonary microvascular endothelial cells and lowest in lymphatic endothelial cells, while CD209L was relatively higher in lymphatic endothelial cells . Conclusions Human microvascular endothelial cells are an important target of SARS-CoV. The damage of pulmonary microvascular endothelial cells and lymphatic endothelial cells may be mediated by two different SARS-CoV receptors respectively.