从抗人膀胱癌改形噬菌体抗体库中筛选出一组相对亲和力常数和细胞结合活性相近的单链抗体 ,而它们的结合活性均明显高于起始的鼠源单链抗体。在亲和力淘选的过程中 ,这一组抗体的表达量存在显著差异 ,这在一定程度上影响到筛选过程中ELISA鉴定的结果 ,使整个筛选过程并不仅仅是对高亲和力抗体的筛选 ,也同时得到了高表达量的抗体。 A panel of anti-human bladder cancer antigen phage antibody library screened a group of relative affinity constants and cell-binding activity of single-chain antibodies, and their binding activity were significantly higher than the initial murine single-chain antibody. In the process of affinity panning, there are significant differences in the expression level of this group of antibodies, which to a certain extent, affect the results of ELISA identification during the screening process, so that the whole screening process is not only screening high-affinity antibodies, At the same time, high expression of antibodies was obtained.