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目的:观察艾叶水提物及其发酵物对白色念珠菌性阴道炎的治疗作用。方法:采用小鼠阴道内接种1×107cfu/mL白色念珠菌悬液20μL,建立白色念珠菌阴道炎模型。将成模小鼠随机分为模型对照组、艾叶水提物组[(生药,下同)20g·L-1]、艾叶发酵物低、中、高剂量组[(生药,下同)10,20,40 g·L-1]及氟康唑组(2 g·L-1),另设空白对照组,每组15只。镜检各组小鼠阴道灌洗液白色念珠菌及脱落上皮细胞,并计算其转阴率,测定阴道灌洗液菌落形成单位(CFU),HE染色观察各组小鼠阴道组织病理改变。结果:模型对照组各小鼠阴道灌洗液均可见白色念珠菌感染,但空白对照组未见。与模型对照组比较,给药后第5,8天艾叶发酵物各剂量组及氟康唑组均能显著增加白色念珠菌孢子及脱落细胞转阴率,降低CFU数量,减轻阴道组织损伤(P<0.05,P<0.01),且艾叶发酵物于给药第8天,中、高剂量组上述指标结果更显著(P<0.01);而艾叶水提物组与模型对照组比较上述各指标无差异。结论:艾叶发酵物对白色念珠菌阴道炎小鼠具有显著治疗作用,艾叶水提物疗效较艾叶发酵物差。
Objective: To observe the therapeutic effect of water extract of fermented leaves and its fermented material on Candida albicans vaginitis. Methods: Candida albicans vaginitis model was established by intravaginal inoculation of 20 μL of 1 × 107 cfu / mL Candida albicans suspension. Mice were randomly divided into model control group, Artemisia argyi aqueous extract group [(crude drug, the same below) 20g · L-1], Artemisia leaf fermented low, medium and high dose group [(crude drug, the same below) 10,20 , 40 g · L-1] and fluconazole group (2 g · L-1), another blank control group, 15 in each group. Microscopic examination of Candida albicans and shedding epithelial cells of vaginal lavage fluid of mice in each group, and calculate the rate of negative conversion. The colony forming units (CFU) of vaginal lavage fluid were measured. The pathological changes of vaginal tissues were observed by HE staining. Results: Candida albicans infection was observed in the vaginal lavage fluid of mice in the model control group, but not in the blank control group. Compared with the model control group, each dose of fermented yar leaf extract and the fluconazole group on the 5th and 8th day after administration significantly increased the negative rate of C. albicans spores and exfoliated cells, decreased the number of CFU, and alleviated vaginal tissue injury (P <0.05, P <0.01), and the results of the above mentioned indicators were more significant (P <0.01) on the 8th, difference. Conclusion: Fermented leaves of Aloe vera have significant therapeutic effects on C. albicans vaginitis mice.