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为了满足普通实验室对茶中茶氨酸测定的需要,研究了茶氨酸的纸层析-分光光度检测方法。结果表明,用茚三酮-乙醇水溶液做展层剂,对茶苗根、芽叶和茶叶的水浸提液进行纸层析,能够有效地将茶氨酸与其它氨基酸分离,紫色色斑清晰而均匀。用乙醇溶液洗脱色斑后用分光光度计在570nm比色,在20～70μL茶氨酸溶液点样量范围内其含量与吸光度呈线性关系。本方法检出限为0·0057mg·mL-1,测定下限为0·0191mg·mL-1,平均回收率90·28%～115·38%,平均相对标准差1·51%,具有安全、药品种类少和操作步骤简单等特点。
In order to meet the needs of the common laboratory for the determination of theanine in tea, paper chromatography - spectrophotometric detection of theanine was studied. The results showed that the ninhydrin - ethanol aqueous solution as a spreading agent, tea seedlings roots, shoots and leaves of tea leaves aqueous extract chromatography, can effectively separate theanine and other amino acids, purple spots clear And even. The color spots were eluted with ethanol and colorimetric with a spectrophotometer at 570nm. The content was linearly related to the absorbance in the range of 20 ~ 70μL Theanine solution. The detection limit of this method was 0 · 0057mg · mL-1, the lower limit of determination was 0 · 0191mg · mL-1, the average recovery was 90.28% -115.38%, with an average relative standard deviation of 1.51% Less types of drugs and simple steps and so on.