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目的:探讨自体骨髓间充质干细胞移植治疗中枢神经系统损伤后神经功能障碍的有效性和安全性。方法:2004年10月~2005年5月,应用自体骨髓间充质干细胞移植治疗5例中枢神经系统损伤后神经功能障碍患者(脑卒中患者3例,脑外伤术后患者2例)。手术首先抽取自体骨髓细胞悬液,在体外经过分离培养后,制成骨髓间充质干细胞悬液,通过静脉输注方式移植脑内。结果:移植治疗后所有患者均未见不良反应。出院半年后随访,最长随访时间达2年。5例患者神经功能缺损症状较移植前均有很大程度的改善,其中2例神志不清患者神志恢复,可按吩咐动作;4例患者肌力提高1~3个级别;失语症状均得到一定改善。结论:自体骨髓间充质干细胞移植治疗中枢神经系统损伤后神经功能缺损疾病是一种简单、安全、有效的方法,值得进一步开展临床观察和验证。
Objective: To investigate the efficacy and safety of autologous bone marrow mesenchymal stem cell transplantation in the treatment of neurological dysfunction after central nervous system injury. Methods: From October 2004 to May 2005, 5 patients with neurological dysfunction after central nervous system injury were treated with autologous bone marrow mesenchymal stem cell transplantation (3 in stroke patients and 2 in patients with traumatic brain injury). Surgery first extracted autologous bone marrow cell suspension after in vitro isolation and culture, made of bone marrow mesenchymal stem cell suspension transplanted by intravenous infusion of the brain. Results: No adverse reactions occurred in all patients after transplantation. After six months of follow-up after discharge, the longest follow-up time of 2 years. The symptoms of neurological deficits in 5 patients were improved to a great extent compared with those before transplantation, of which 2 patients recovered unconsciously and were able to act according to instructions. The muscle strength of 4 patients increased by 1 to 3 levels and the symptoms of aphasia were all fixed improve. CONCLUSION: Autologous bone marrow mesenchymal stem cell transplantation is a simple, safe and effective method for the treatment of neurological deficit after central nervous system injury. It is worth further clinical observation and verification.