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[目的]探讨白色念珠菌免疫小鼠的T细胞、腹腔巨噬细胞和免疫血清,在抗小鼠全身性白色念珠菌感染中各自所起的作用。[方法]将60只健康昆明种小鼠随机分组,分别进行下述试验。(1)体内试验:将致敏T细胞、腹腔巨噬细胞、免疫血清分别过继给同品系小鼠后,尾静脉注射0.2ml白色念珠菌液(2×107CFU/ml),观察小鼠存活情况;(2)体外试验:将上述细胞、免疫血清分别与0.1ml白色念珠菌液(1×105CFU/ml)共同培养,计数菌落形成单位(CFU),判定细胞和免疫血清对白色念珠菌的作用效应。[结果](1)感染后第4周,过继腹腔巨噬细胞和致敏T细胞组小鼠存活率分别为50%和60%;过继免疫血清和生理盐水组小鼠全部死亡;(2)体外实验发现腹腔巨噬细胞对白色念珠菌具有明显抑杀作用,菌落数(2.33±0.58×104CFU/ml)显著低于对照组(9.33±2.51×104CFU/ml),而免疫血清组菌落数(76.00±3.60×104CFU/ml)显著高于对照组。[结论]小鼠巨噬细胞在抗白色念珠菌感染中发挥重要作用,免疫血清没有保护作用。
[Objective] To investigate the role of T cells, peritoneal macrophages and immune sera of Candida albicans immunized mice in their anti-mouse systemic Candida albicans infection. [Method] Sixty healthy Kunming mice were randomly divided into the following experiments. (1) In vivo test: After sensitized T cells, peritoneal macrophages and immune sera were respectively passed on to the same strain of mice, 0.2 ml Candida albicans solution (2 × 10 7 CFU / ml) was injected through the tail vein to observe the survival of the mice ; (2) In vitro experiments: The above cells and immune sera were co-cultured with 0.1 ml Candida albicans solution (1 × 10 5 CFU / ml), and the colony forming units (CFU) were counted to determine the effect of the cells and immune serum on Candida albicans effect. [Results] (1) The survival rate of mice in adoptive intraperitoneal macrophages and sensitized T cells was 50% and 60% respectively in the 4th week after infection; all the mice in adoptive immunized and normal saline groups died; (2) In vitro experiments showed that peritoneal macrophages had a significant inhibitory effect on Candida albicans, the number of colonies (2.33 ± 0.58 × 104CFU / ml) was significantly lower than that of the control group (9.33 ± 2.51 × 104CFU / ml), while the number of colonies 76.00 ± 3.60 × 104CFU / ml) was significantly higher than the control group. [Conclusion] Mouse macrophages play an important role in anti-Candida albicans infection, while immune serum has no protective effect.