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目的 以白蛋白( Alb) m RNA 作为肝细胞在外周血中的标志物,从而早期检出血中的微量肝癌细胞。方法 用RTPCR 方法检测20 例正常人,10 例急、慢性肝炎,10 例肝硬化患者及39 例原发性肝癌(HCC) 患者外周血中的白蛋白m RNA 。结果39 例原发性肝癌( HCC) 患者中有12 例呈阳性,其中8 例经临床证实为有转移的患者中,6 例Alb m RNA 为阳性,阳性率为75 .0 % ,其它31 例临床上没有明显转移的患者中,有6 例Alb m RNA 阳性,阳性率为19 .3 % 。10 例急、慢性肝炎患者中,仅有1 例为阳性。20 例正常人和10 例肝硬化患者均呈阴性。结论 有转移的HCC 患者与无转移的HCC 患者的外周血中Alb m RNA 的阳性率具有显著性差异,HCC 患者外周血中Alb m RNA 为阳性,提示癌细胞已进入血循环,肝外转移危险性大。
Aim To use albumin (Alb) m RNA as a marker of liver cells in peripheral blood to detect early traces of liver cancer cells in the blood. Methods RT-PCR was used to detect albumin mRNA in peripheral blood of 20 normal subjects, 10 acute and chronic hepatitis, 10 cirrhosis patients and 39 primary hepatocellular carcinoma (HCC) patients. Results Of the 39 cases of primary hepatocellular carcinoma (HCC), 12 were positive, of which 8 were clinically proven to be metastasized and 6 were positive for Alb m RNA with a positive rate of 75. 0%. Of the other 31 patients with no clinically significant metastases, 6 were positive for Alb m RNA with a positive rate of 19. 3%. Only 10 of 10 acute and chronic hepatitis patients were positive. 20 normal subjects and 10 patients with cirrhosis were negative. Conclusion The positive rate of Alb m RNA in peripheral blood of patients with metastatic HCC and non-metastatic HCC has significant difference. The positive expression of Alb m RNA in peripheral blood of patients with HCC suggests that cancer cells have entered the blood circulation and the extrahepatic metastasis risk Big.