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八角(Illicium Verum Hook·f)又名八角茴香、大茴香、大料是著名调味香料。其果皮、种籽及叶含芳香油,其主要成分是茴香醚(anethole)等。但在香油中含有黄樟醚(Safrole),据报导,黄樟醚对肝脏有一定毒性,且是一种前致癌物。为此,我们对八角茴香提出的香油进行了鼠伤寒沙门氏菌营养缺陷型回复突变试验(Ames),现报告如下。一.材料和方法 1.材料 (1)样品:八角茴香系广西桂林植物研究所植物园(栽培)提供的干果。将其置于60℃烤箱烘干后,碾成粗粉,经水蒸汽蒸馏后得到挥发油成分,含量约5％。(2)菌株:采用TA98移码突变型菌株及TA100碱
The octagon (Illicium Verum Hook·f), also known as star anise, anise, and aniseed, is a famous spice. Its pericarp, seeds and leaves contain aromatic oils. Its main component is an anethole. However, Safrole is contained in sesame oil and it has been reported that Scutellarin is toxic to the liver and is a pre-carcinogen. To this end, we carried out the anaerobic back-mutation test (Ames) of Salmonella typhimurium on the sesame oil proposed by the star anise. The report is as follows. 1. Materials and Methods 1. Materials (1) Samples: Star anise is the dried fruit provided by the Botanical Garden of Guilin Institute of Botany (Guangxi). After being dried in an oven at 60°C, the mixture was ground into a coarse powder and steam distilled to obtain a volatile oil content of about 5%. (2) Strain: TA98 frameshift mutant strain and TA100 base