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目的探讨大鼠脑震荡后不同时间大脑多巴胺D1受体在不同部位的表达变化。方法用金属单摆打击装置复制大鼠脑震荡模型,将56只大鼠随机分为脑震荡后1、2、4、8、16和24d 6个损伤组和1个正常对照组,每组8只。于大鼠脑震荡后不同时间取内侧隔核(MSN)、左右Broca斜角带垂直支(VDB)、丘脑网状带(RT)脑组织,用免疫组化染色(SP法)观察脑组织不同区域多巴胺受体(D1)表达情况,用图像分析系统进行定量分析。结果各组大鼠脑组织D1受体免疫组化阳性表达变化情况:在MNS区,脑震荡后1d细胞阳性表达开始减弱,到16d细胞阳性表达最弱,16～24d细胞阳性表达开始增强,但在24d仍比对照组的表达弱。在VDB区,脑震荡后1d细胞阳性表达开始减弱,2d细胞阳性表达最弱,脑震荡后2～24d细胞阳性表达开始增强,但在24d仍比对照组的表达弱。在RT区,脑震荡后1d细胞阳性表达开始减弱,4d细胞阳性表达最弱,4～24d细胞阳性表达开始增强,到24d与对照组表达接近。结论大鼠脑震荡后不同时间,脑组织多巴胺D1受体在MSN、VDB、RT不同区域的表达轻微下调,但下调差异无显著性意义。
Objective To investigate the changes of dopamine D1 receptors in different parts of the brain at different time after concussion in rats. Methods The rat model of concussion was replicated with a metal pendulum striking device. 56 rats were randomly divided into six injury groups (1, 2, 4, 8, 16 and 24 days after concussion) and a normal control group only. The medial septum (MSN), left and right brachial diaphragmatic (VDB) and thalamic reticular band (RT) brain tissues were taken at different time points after brain concussion. The brain tissues were observed by immunohistochemistry (SP method) Regional dopamine receptor (D1) expression, using image analysis system for quantitative analysis. Results The expression of D1 receptor immunohistochemical changes in brain tissue of rats in each group changed gradually. In MNS area, the positive expression began to weaken on the 1st day after concussion, the expression was weakest on the 16th day, and began to increase on the 16th to 24th day, The expression was still weaker than the control group at 24 days. In the VDB area, the positive expression began to diminish on the 1st day after concussion and the weakest on the 2nd day. The positive expression began to increase at 2 ~ 24th day after concussion, but was still lower than that of the control group at 24th day. At RT, the positive expression of cells began to diminish one day after concussion, the expression was weakest on day 4, and began to increase on day 4 to 24, reaching to the level of control at 24 days. Conclusions The expression of dopamine D1 receptor in brain tissue at different time points after the brain concussion was slightly down-regulated in different regions of MSN, VDB and RT, but the difference was not significant.