We report the experimental observation of two-dimensional Talbot effect when a resonance plane wave interacts with a two-dimensional atomic density grating generated by standing wave manipulation of ultracold Bose gases.Clear self-images of the grating and sub-images with reversed phase or fractal patterns are observed. By calculating the autocorrelation functions of the images, the behavior of periodic Talbot images is studied. The Talbot effect with two-dimensional atomic density grating expands the applications of the Talbot effect in a wide variety of research fields. We report the experimental observation of two-dimensional Talbot effect when a resonance plane wave interacts with a two-dimensional atomic grating generated by standing wave manipulation of ultracold Bose gases. Clear self-images of the grating and sub-images with reversed phase or By calculating the autocorrelation functions of the images, the behavior of periodic Talbot images is studied. The Talbot effect with two-dimensional atomic density diffraction expand the applications of the Talbot effect in a wide variety of research fields.