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采用双抗夹心EIASA法测定30例哮喘发作期、12例哮喘缓解期患儿血浆及外周血单个核细胞(PBMC)白细胞介素4(IL-4)、白细胞介素12(IL-12)与血清IgE的水平,结果表明:哮喘患儿发作期、缓解期血清IgE水平、血浆及PBMC经PHA和LPS刺激后的IL-4水平较正常对照组明显升高(P<0.01),IL-12水平较正常对照组明显降低(P<0.01)。且缓解期IgE、IL-4及IL-12水平较发作期均有明显差异(P<0.05)。提示哮喘患儿发作期及缓解期均存在IgE、IL-4及IL-12水平失衡。
The levels of IL-4, IL-12 and IL-12 in plasma and peripheral blood mononuclear cells (PBMC) in 12 asthmatic children with asthma were determined by EIASA method. Serum IgE levels in children with asthma were significantly higher than those in normal control group (P <0.01). The levels of IL-12 in plasma and PBMC after PHA and LPS stimulation were significantly higher than those in normal control group Compared with the normal control group, the level was significantly lower (P <0.01). The levels of IgE, IL-4 and IL-12 in remission stage were significantly higher than those in attack stage (P <0.05). Prompted the onset of asthma in children and remission are the existence of IgE, IL-4 and IL-12 levels imbalance.