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为探明大麦品种的不同氮利用效率差异及质体型谷氨酰胺合成酶基因的表达差异,以48份大麦品种为材料,并利用不同氮水平营养液水培8周,研究其在低氮(0.4 mmol·L~(-1))、中氮(2.0 mmol·L~(-1))及高氮(5.0 mmol·L~(-1))下根、茎、叶的相关性状,筛选氮高效利用和氮低效利用的大麦品种,并利用qRT-PCR对筛选到的大麦氮高效和氮低效品种的GS2基因表达特性进行分析。结果表明,中氮下,除根干重和根含氮量外,其余的大麦苗期根、茎、叶各生物性状在品种间差异极显著,根据不同氮处理下的48份大麦品种苗期性状以及氮利用效率,筛选到1个氮高效品种(Z0099001)和2个氮低效品种(ARr-91和甘啤7号)。3个氮处理水平下,氮高效品种GS2基因的相对表达量高于氮低效品种,并且氮高效品种和氮低效品种根、茎、叶中GS2基因的相对表达量也各不相同,其中,叶中最高,茎中次之,根中最低。低氮条件下,根茎叶中,Z0099001的GS2基因的相对表达量高于甘啤7号,ARr-91最低。中氮条件下,根、茎、叶中均表现为氮高效品种高于氮低效品种,但在根中差异不显著,茎和叶中差异显著。高氮条件下,3个品种在根中GS2基因的相对表达量较低;在茎和叶中,Z0099001的相对表达量显著高于ARr-91和甘啤7号,且Z0099001叶中GS2基因的相对表达量在所有性状中最高。本研究结果为进一步解析大麦氮高效利用率奠定了基础。
In order to find out the difference of nitrogen use efficiency and the difference of expression of plastid glutamine synthetase among different barley cultivars, 48 barley cultivars were used as materials, 0.4 mmol·L -1, 2.0 mmol·L -1 and 5.0 mmol·L -1, respectively, and the relative contents of nitrogen Efficient use of barley varieties with low nitrogen use efficiency and qRT-PCR analysis of the GS2 gene expression characteristics of the selected barley high nitrogen and nitrogen inefficient varieties. The results showed that the root, stem and leaf of the remaining barley were significantly different in all varieties except the root dry weight and the root nitrogen content under medium nitrogen. According to the seedling traits of 48 barley cultivars under different nitrogen treatments And one nitrogen efficient variety (Z0099001) and two nitrogen inefficient varieties (ARr-91 and Ganpi No.7) were screened. Under the three nitrogen treatments, the relative expression level of GS2 gene in nitrogen-efficient variety was higher than that in nitrogen-inefficient variety, and the relative expression amount of GS2 gene in root, stem and leaf of nitrogen-efficient variety and nitrogen-inefficient variety was also different, , The highest in leaves, the second in stems, the lowest in roots. Under low nitrogen conditions, the relative expression of GS2 gene in Z0099001 was higher than that in Ganpi 7, and the lowest in ARr-91. Under medium nitrogen conditions, the nitrogen efficient varieties were higher in roots, stems and leaves than the nitrogen inefficient ones, but the differences were not significant in roots and significant differences in stems and leaves. Under high nitrogen conditions, the relative expression level of GS2 gene in the three cultivars was lower than that in other two cultivars. The relative expression of Z0099001 in stems and leaves was significantly higher than that in ARr-91 and Ganpi 7, and the GS2 gene in Z0099001 The relative amount of expression is highest among all traits. The results of this study laid the foundation for further analysis of efficient utilization of nitrogen in barley.