将正常和糖尿病模型雌鼠与正常雄鼠交配,经阴道涂片检查确定是否妊娠。在妊娠第21或22天处死大鼠,取出子宫,分离胎儿和胎盘,将对照组和糖尿病组子宫切成约10mm长条,放入含20mlKRB氏液的组织浴中并与拉力换能器连接以测定子宫等长收缩(IDT)。用放射免疫法测廿烷类含量。用液闪仪测U~(14)C-葡萄糖氧化释出的~(14)CO_2。含量以确定葡萄糖氧化作用。用分光光度计测糖原含量。甘油三酯根据McFayden方法测定。结果:①廿烷含量:6-酮 Normal and diabetic female mice were mated with normal males, vaginal smear examination to determine whether the pregnancy. The rats were sacrificed on day 21 or 22 of gestation, the uterus was removed, the fetus and placenta were separated and the uteri of the control and diabetic uteri were sectioned into strips approximately 10 mm into a tissue bath containing 20 ml of KRB solution and connected to a pull transducer To determine uterine isometric contraction (IDT). Determination of eicosane by radioimmunoassay. The ~ (14) CO_2 released by the U ~ (14) C-glucose oxidation was measured by liquid scintillation. Content to determine glucose oxidation. Spectrophotometer with glycogen content. Triglycerides were determined according to McFayden’s method. Results: ① eicosane content: 6-ketone