[目的]研究香蕉胚性悬浮细胞系建立过程中非胚性成分的去除方法。[方法]以巴西蕉的花蕾进行诱导培养后,运用孔径在250~500μm的不锈钢筛网进行过滤,并用倒置显微镜对培养物进行实时观测。[结果]运用孔径在250~500μm的不锈钢筛网能够有效去除悬浮细胞系中的褐化坏死组织和分生组织小球,但如果在悬浮细胞系诱导后3 d立即进行去除,则易使之后胚性细胞的增殖变得困难;如果在悬浮细胞系诱导后14 d左右去除,则褐化坏死组织释放的酚类物质很容易损伤并杀死正在增殖的胚性细胞。悬浮细胞系诱发后7 d是一个比较恰当的过滤去除时间点。[结论]在培养的过程中,将悬浮细胞系定期放置在倒置显微镜下进行观察,用移液器能将液泡化的细胞和胚去除。在操作前,需将倒置显微镜放置在超净工作台内,用紫外线长时间照射进行表面灭菌。这样即可以将悬浮细胞系中的非胚性成分过滤去除,又能预防感染。 [Objective] The study aimed to study the removal of non-embryogenic components during the establishment of banana embryogenic suspension cell lines. [Method] The buds of Brazil banana were induced and cultured, then filtered using a stainless steel mesh with a diameter of 250 ~ 500μm, and the culture was observed by inverted microscope in real time. [Result] The browning necrotic tissue and meristematic pellet in suspension cell line could be effectively removed by the stainless steel mesh with the diameter of 250 ~ 500μm. However, if it was removed immediately after 3 days of induction of suspension cell line, Proliferation of embryogenic cells becomes difficult; if it is removed about 14 days after induction by the suspension cell line, the phenolic substances released from the browning necrotic tissue can easily damage and kill the proliferating embryogenic cells. Seven days after the suspension cell line was induced, it was a more appropriate filter removal time point. [Conclusion] During the culture process, the suspension cell lines were periodically placed under an inverted microscope for observation, and vacuolar cells and embryos were removed by pipette. Before operation, place the inverted microscope in a clean bench and sterilize the surface with ultraviolet radiation for a long time. In this way, the non-embryogenic components in the suspension cell line can be filtered and removed, and the infection can be prevented.