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目的:建立体外培养SD大鼠小脑神经元划痕模型。方法:用200μl塑料枪头划痕体外培养大鼠小脑神经元,观察相同视野下不同时间点(0,24,48 h)的划痕宽度。结果:倒置相差显微镜下可见划痕细胞后0 h即形成划痕区,边缘整齐。24 h后,划痕边缘变模糊同时划痕宽度变窄。48 h后,划痕宽度进一步缩小。结论:本模型简便经济,能较好地模拟脑损伤后神经元的迁移。
Objective: To establish a rat model of cerebellar neuronal scratching in vitro. Methods: The neurons of rat cerebellum were cultured in vitro with 200μl plastic scratches and the width of scratches at different time points (0, 24, 48 h) under the same field of vision were observed. Results: The scratched area was formed at 0 h after inverted phase contrast microscopy and the edges were neat. After 24 h, the edges of the scratches became blurred and the width of the scratches became narrower. After 48 h, the scratch width was further reduced. Conclusion: This model is simple and economical, and can better simulate the migration of neurons after brain injury.