Based on the measurements from 52 Chinese subjects (26 males and 26 females), a high-spatial-resolution head-related transfer function (HRTF) database with corresponding anthropornetric parameters is established. By using the database, cues relating to sound source localization, including interaural time difference (ITD),interaural level difference (ILD), and spectral features introduced by pinna, are analyzed. Moreover, the statistical relationship between ITD and anthropometric parameters is estimated. It is proved that the mean values of maximum ITD for male and female are significantly different, so are those for Chinese and western subjects. The difference in ITD is due to the difference in individual anthropometric parameters. It is further proved that the spectral features introduced by pinna strongly depend on individual; while at high frequencies (f ≥ 5.5 kHz), HRTFs are left-right asymmetric. This work is instructive and helpful for the research on binaural hearing and applications on virtual auditory in future.