论文部分内容阅读
目的探讨不同浓度的雷洛昔芬(raloxifene,RAL)对体外培养的人主动脉瓣间质细胞(AVICs)增殖和凋亡的影响。方法用Ⅱ型胶原酶裂解主动脉瓣膜,获得AVICs并进行传代培养,使用不同浓度(0 nmol/L、0.1 nmol/L、1 nmol/L、10 nmol/L、100 nmol/L、1 000 nmol/L)的RAL作用于AVICs细胞,其中0 nmol/L RAL组作为对照组,其余为实验组,培养0 d、3 d、5 d、7 d、9 d后,采用细胞增殖与毒性检测试剂盒(MTS)检测不同浓度的RAL对AVICs增殖的影响,收集第7 d的细胞用流式细胞仪检测细胞的周期和凋亡。结果与对照组相比,MTS检测结果表明10 nmol/L RAL和100 nmol/L RAL浓度组的细胞于第5、7、9 d在490 nm波长处的吸光度值(OD值)明显降低(P<0.05),收集第7 d的细胞,流式细胞检测仪检测结果显示,10 nmol/L和100 nmol/L浓度组RAL能明显降低AVICs细胞周期中S期比例(P<0.05)和细胞凋亡率(P<0.05)。结论合适浓度的RAL具有抑制AVICs增殖和凋亡的作用,为进一步研究RAL对主动脉瓣病变的作用奠定了基础。
Objective To investigate the effects of different concentrations of raloxifene (RAL) on the proliferation and apoptosis of cultured human aortic valve interstitial cells (AVICs). Methods Aortic valves were lysed by type Ⅱ collagenase and AVICs were obtained and subcultured. The cells were treated with different concentrations (0 nmol / L, 0.1 nmol / L, 1 nmol / L, 10 nmol / L, 100 nmol / L, / L) was used to treat AVICs cells. The RAL group (0 nmol / L) was used as the control group, and the rest was the experimental group. After cultured for 0 d, 3 d, 5 d, 7 d and 9 d, cell proliferation and toxicity assay (MTS) was used to detect the effect of different concentration of RAL on the proliferation of AVICs. The cells on the 7th day were collected to detect the cell cycle and apoptosis by flow cytometry. Results Compared with the control group, the MTS results showed that the absorbance values (OD values) at 490 nm of the 5th, 7th and 9th day were significantly lower in the 10 nmol / L RAL and 100 nmol / L RAL groups (P (P <0.05). The cells collected on the 7th day were detected by flow cytometry. The results showed that RAL at 10 nmol / L and 100 nmol / L significantly decreased the proportion of S phase (P <0.05) Mortality (P <0.05). Conclusion RAL with the proper concentration can inhibit the proliferation and apoptosis of AVICs, which lays the foundation for further study on the effect of RAL on aortic valve disease.