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目的建立减肥类保健食品中非法添加酚酞的薄层色谱-傅里叶变换红外光谱(TLC-FTIR)联用检测方法。方法样品用无水乙醇超声提取,固定相为GF254铝合金硅胶板(10cm×20cm),展开剂为乙酸乙酯∶石油醚(60～90)∶甲醇=10∶6∶1,点样量为2μl,分别用紫外灯254nm和自制的2%NaOH试纸两种检视方法初筛,再利用薄层色谱制备技术将可疑斑点分离纯化后进行FTIR检测,并与对照品的FTIR谱图比较确认。结果检测的10批样品中有5批样品含有酚酞,与采用薄层扫描法和高效液相色谱法的检测结果一致。结论所建立的方法准确、可靠,可作为该类产品是否添加酚酞的检测方法。
Objective To establish a thin-layer chromatography-Fourier transform infrared spectroscopy (TLC-FTIR) combined detection method for illegally adding phenolphthalein in dietary supplements. Methods The sample was extracted with anhydrous ethanol by ultrasonic. The stationary phase was GF254 aluminum alloy silica gel plate (10cm × 20cm). The developing solvent was ethyl acetate: petroleum ether (60-90): methanol = 10: 2μl, UV lamp 254nm and homemade 2% NaOH test paper two kinds of screening method for screening, and then using thin-layer chromatography preparation of the suspicious spots were isolated and purified by FTIR detection and comparison with the FTIR spectra confirmed. Results Five of the 10 batches tested contained phenolphthalein, consistent with the results of TLC and HPLC. Conclusion The established method is accurate and reliable and can be used as a detection method of phenolphthalein for such products.